Cytological Analysis Of The Progeny From Triticum Timopheevi × Avena Fatua L. Var. Glabrata Pat

The chromosomic meiotic behavior of pollen mother cells(PMCs) of the progeny from Triticum timopheevi × Avena Fatua L. Var. glabrata Pat was researchhed by conventional cytological analysis and sequential C-banding-GISH in the present study. The main results were as follows.Cytological analysis showed that a large number of univalents, lagging chromosomes, chromosome bridges and micronuclei were found at metaphase I, anaphase I, anaphaseⅡand tetrad stages in these progeny, indicating considerably genetic instability.Sequential C-banding-GISH analysis revealed that some Avena Fatua L. Var. glabrata Pat chromotin was detected at metaphase I and anaphase I. Sequential C-banding-GISH analyses at metaphase I and anaphase I of the TGF1 generation showed red short signals, which should derive from the paternal chromosomes The chromosomes with the hybridization signals were the chromosomes 2A, 3G, 6G and 7G. Sequential C-banding-GISH analyses at metaphase I and anaphase I of the TG F2 generation showed similar red short signals in the chromosomes 2A, 3G, 6G and 7G. But the signals in the chromosomes 2A and 3G might be lost in some plants due to the inharmony between the genomes of two parents. In addition, partial T. timopheevii chromosomes appeared in the form of lagging chromosomes, which might result from large genome differences between the two parents, and the wild oat chromosome introgression interfered with the wheat homologues’ normally pairing.