Genetic Diversity Of AM Fungi In Rhizosphere Soil Of Ammopiptanthus Mongolicus

The symbiosis with AM fungi provides plants tolerance to environmental stress, promotes plant growth and keep the diversity and stability of plants community. This ancient symbiotic relationship plays a key role in the establishment of land plants. Ammopiptanthus mongolicus is a broad-leaved evergreen plant which has significant effects in windbreak and sand fixation. With the increasingly serious desertification, A.mongolicus has being an important plant for desert afforestation and vegetation restoration and AM fungi were used in the introduction and transplantation of A.mongolicus.Recently researches have focusing on the drought resistant gene of A.mongolicus, but little about AM fungal communities and genetic diversity of its rhizosphere soil. To elucidate the features of AM fungal communities and the genetic diversity of AM fungi in different sites of A.mongolicus, we sampled different depth rhizosphere soil of A.mongolicus from four sites in the desert of Mongolia and molecular method was used. The following results were obtained.1. AM1/NS31 is the best primer to study the communities of AM fungi in A. mongolicus rhizosphere soil of four chose specific primers.2. Using molecular method we identify Rhizophagus intraradices and Glomus indicum which are not found through morphological method. The similarity of AM fungi species composition of different sites and soil depths is up than 50%, however, there are significant differences at the dominance of different soil samples. The dominant species of both two sites are Glomus and Rhizophagus.3. Soil depth significantly influent the richness and diversity of AM fungi. The richness and diversity are much higher at 0-20 cm layer than at 20-40 cm layer.4. RDA analysis revealed that soil PH had a significantly negative correlation with AM fungi Shannon-Weiner and species richness. Available P, available N, available K and organic had a significantly positive correlation with AM fungi Shannon-Weiner index, species richness.5. Phylogenetic analysis showed that it’s much closer relationship in F. mosseae and Funneliformis than in F. mosseae and Glomus.6. There is little difference in 18 Sr RNA of five kinds of AM fungi in rhizosphere soil of A.mongolicus. Compare the distances of F mosseae in desert, farmland, forest and preservation environment, we found the distance of F mosseae of different sites in desert environment is significant larger than it in four environments.