Study On Seed Optimum Germination Conditions And Rapid Propagation Technology Of Astilboides Tabularis

This study with Astilboides tabularis experimental material,study on seed optimum germination conditions of Astilboides tabularis for the first time,the effects on seed germination about low temperature stratification treatment time, seed handling, the selection of germination bed temperature and light conditions.Rapid propagation technology of Astilboides tabularis,and discovered Astilboides tabularis can be propagated as “ breeding seedlings with seedlings” in the process,get lots of rootless seedlings through multiple shoot clumps induction training.Then as immature embryo and sterile petiole of Astilboides tabularis for explant,for in vitro rapid propagation technology research of Astilboides tabularis,establish the asexual reproduction system of Astilboides tabularis successfully,and discuss the influence of in vitro rapid propagation cultivation of Astilboides tabularis such as inorganic salt content, kinds of plant hormones and ratio, concentration of sucrose, activated carbon added, etc.Multiple comparison analysis was carried out on the test results with DPSv7.05 software,experiment is mainly the following results are obtained:1.The Astilboides tabularis seed has a physiological after ripening phenomena, cold stratification can significantly improve the germination rate,shorten the time of germination,after 90 days laminated processing,the germination rate increased from 44.67%to 70.33%,emergence is neat;Cryogenic treatment seriously affected the normal seed germination;It is favorable for seed germination by 40℃seed soaking in warm water,the germination rate increased from 68%to 76.33%;Seeds with the concentration of GA3 after 400mg/L treatment to get the highest germination rate,from 71.33%to 93.33%,while the NAA and 6-BA on seed germination inhibition.Seed germination medium and filter paper as Astilboides tabularis seed optimum germination bed,germination rate reached 75%and 72%respectively;The Astilboides tabularis seeds is light like seeds,the optimum light conditions for periodic illumination(13h/d),the germination rate was 72%,3.18 times the total darkness;variable temperature treatment is more advantageous to Astilboides tabularis seed germination,15℃/25℃is the the highest in the treatment,the germination rate was 75.33%;In conventional breeding,the mixed matrix of garden soil,peat and sand is the optimal matrix of seed germination,the germination rate was 71.33%.2.The optimum sterilization method of Astilboides tabularis seed that the sterilization combination with 75%alcohol for 30 s and 0.1%HgCl2 for 4min,the contamination rate was 2.67%and the germination rate was 74.15%;Influence of kinds of culture medium on Germination of the seeds had no significant difference,but has greater effect on the seed germination of seedlings after growth,the highest average seedling height is 2.34 cm in MS medium;Sucrose content have certain influence on seed germination and seedling growth,sucrose content of 30g/L was appropriate;The content of inorganic salts on the growth of seedlings is important,reduction of inorganic salt content is not conducive to the growth of the seedlings,the optimum medium for seedling culture is MS medium,and in the seedling breeding,try to choose a height greater than 3cm seedlings.The optimum medium is MS+1.0 mg/L 6-BA+0.1 mg/L NAA,the proliferation coefficient and shoot length reached the highest,respectively 6.27 and 4.23 cm,the bud is grow robustly and the leaf color became dark green.The kinds of culture medium had no significant effect on rooting rate,the rooting rate reached 100%,1/2MS medium was the optimum medium for adventitious bud rooting,root number and average length of root respectively 8.53 and 4.83 cm.3.The optimum sterilization method of Astilboides tabularis capsule that the sterilization combination with 75%alcohol for 30 s and 0.1%HgCl2 for 4min;Through the cultivation of 60 days,the best explant of callus induction is the seed and sterile;The optimum medium of callus induction of seed is MS+0.5 mg/L 6-BA+1.0 mg/LNAA,the induction rate was 97.24%,the callus of induction was embryogenic calli showed yellow green loose granular;The optimal medium for callus induction of aseptic is MS+0.3mg/L6-BA+ 0.1mg/LNAA,the induction rate was 92.33%,the callus of induction was embryogenic calli showed green loose globular.Differentiation of adventitious buds from Callus Culture:Through the cultivation of 60 days,callus differentiation out of a large number of adventitious bud,the optimum medium of differentiation of adventitious buds from callus is MS+1.5mg/L 6-BA+0.05 mg/L IBA+1.0 mg/LGA3,differentiation rate was 91.67%,growing well.Through the cultivation of 50 days,also risen adventitious bud formation of multiple shoot clumps,the optimum medium not bud proliferation is MS+0.5 mg/L 6-BA+0.2 mg/L IAA,the proliferation rate was 96.67%and the proliferation coefficient was 5.34.4.Through the cultivation of 50 days,rooting effect is good,the optimal medium for rooting is MS+0.5 mg/L IAA+0.5 mg/L GA3,sucrose concentration was 30g/L,rooted plantlets do not need to add active carbon,In the medium of rooting rate reached 98.33%,the average root number 10.24,the average root length is 5.77 cm,the growth state of tissue culture seedlings and root,root number and root quality is the best.5.Seedling training and transplanting:The sealing film is opened and put into a small amount of water,out in the scattering light after 3days,wash the medium with 20±2℃ water,transplanting to the mixed matrix about garden soil:Sand:peat=1:1:1,natural light shading rate of 85%shading,every ventilation and opens the film after 10 days;until the seedlings grow new leaves after 20 days later,it can be increase the light gradually and conduct the normal management of fertilizer and water every day.