Pleiopetalous Flower Physiological Characteristics And Respones Mechanism Of Ethylene Response Factor In Non- Heading Chinese Cabbage

Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), Cruciferae Brassica, is originated in China, and is popular one of the most favorite vegetables. In recent years, the demand of non-heading Chinese cabbage is growing. At present the main cultivated varieties is relatively single. It is the toughest and the most urgent task for the breeders to breed fine cultivars of non-heading Chinese cabbage. Non-heading Chinese cabbage flower development has important effects on reproduction, thus to Non-heading Chinese cabbage flower development in related research is of great significance for breeding new varieties.In this paper, we studied materials that are non-heading Chinese cabbage pleiopetalous flower W051, control W052 and maintain line W053. We Study the characteristics of pleiopetalous and relations with ethylene metabolism. The results were summarized as follows:1. Non-heading Chinese cabbage pleiopetalous flower mutation, control and maintain line the growth of physiological characteristics analysisDifferent material growth indicators measure and take pictures, Repeat 5 times. We observe the early flowering the property of inflorescence, flower and flower bud, and the leaves and buds of chlorophyll and malondialdehyde content were determined.The results show that pleiopetalous flower material showed two types:stamen-petalody and flowers in the flower. The main inflorescence bud number:the control> maintain line > mutation line. Maintain line in flower diameter, stamen length and petal length are significantly larger than control and mutations line. The mutant and control of early budding period and flowering 7 days later than the maintainer line to the left. Mutantion line in main inflorescence are significantly more than the control and maintain line, number of stamens and single flower petal number remains the same. Flowers in flower are produced by the ovary enlargement, new bud burst out more. Flower buds of chlorophyll: mutantion line> control> maintainer line, and various types of chlorophyll in the leaves: maintainer line> mutation line> control. Buds of malondialdehyde in mutation is far higher than control and maintainer line, control and maintainer line respectively 31.9 times and 36.6 times. Mutation of malondialdehyde content in the leaf is also significantly higher than that of maintainer line, but no significant difference between mutant and control. These results suggest that mutations line, control and maintainer line show significant difference in growth traits of bud and inflorescence. Mutation line showed premature aging tendency, and we had to the height of the organs of ethylene response way express phenomenon.2. The ethylene response factors influencing the expression in pleiopetalous flower mutant.Firstly, with gas chromatography to detect mutation line、control and maintainer line three non-heading Chinese cabbage organs ethylene release quantity; To measure the organs key restriction enzyme of ethylene biosynthesis of the activity of ACS and ACO; And then using ethephon and exogenous ethylene biosynthesis inhibitors after spraying, real-time fluorescence quantitative detection leaf’s ethylene response factors ERFB001_ACO1 and ACS6 gene expression in 24h; Finally, the flower in the flowering period observation statistics character, and to different spray’s bud ERFB001、ACO1 and ACS6 gene quantitatively expression.The results showed that the mutant release ethylene content in different organs, key restriction enzyme of ethylene biosynthesis of ACS and the ACO activity is significantly higher than control and maintainer line. These exogenous substances after spraying, different materials of parts have obvious variation in bloom. ERFB001 in bud like ACS6 relative expression, all show the Ag+ and Co2+ solution spraying mutation is significantly increased, while other value is very small. This suggests that the mutant promoting ethylene biosynthesis. Silverions to the ACC oxidase gene expression without regulation; Cobaltion suppression before promoting the ACC oxidase gene expression; Silverions and cobaltions have a regulatory role of ACC synthase gene; Silverions and cobaltions are has inhibitory effect on ERFB001 gene expression, and their inhibitory effect on phase. Ethephon inhibit ACC oxidase and ACC synthase gene expression; In Control ethephon inhibit ethylene response factors ERFB001 expression; In maintainer line in the high concentration of exogenous ethylene suppress ERFB001 gene expression, and low concentration of exogenous ethylene to promote ERFB001 gene expression. It is closely related to the ethylene response factors ERFB001 and ACS6 gene, may be the upstream and downstream genes. The Ag+ and Co2+ can significantly increase the bud of ACC synthase gene and ethylene response factors ERFB001 gene expression.3. The flower development related genes in non-heading Chinese cabbage buds and bud mutation of parts of the expressionExperiment test real-time fluorescence quantitative detection non-heading Chinese cabbage buds and each part of flower bud AP2 gene of A category and AG gene of C category expression. Fluorescence quantitative detection of ethylene response factors ERFB001 in ethylene biosynthesis pathway and the key restriction enzyme gene expression in style and flower petals of buds.These results indicated that A gene class may not participate in mutant, C gene class in flowers in the flower mutant pathway may plays an important role. Mutation ethylene response factort ERFB001 is closely related to male sterility characteristic, and mutation has little to do with style. Mutant mutations, ethylene biosynthesis have been improved, reflect the mutations’mutant and the of ethylene response mechanism are closely related.