Characterization And Functional Analysis Of BcCAMTA Transcription Factor Family In Non-heading Chinese Cabbage

Non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino)is a native vegetable in China,which is kind of Chinese cabbage.They both belong to Brassica.Non-heading Chinese cabbage originated in the lower reaches of the Yangtze River in Taihu Lake area,which has a long history of cultivation and has been widely planted.In the long-term domestication and cultivation,the rich germplasm resource has been shaped.With the characteristics of nutrient,easily cultivation,edible petioles and thick leaves,non-heading Chinese cabbage have been widely planted in China and Southeast Asia.With the continuous progress of research,some of them have been introduced to Europe and the United States.However,current research on the molecular biology mechanism of non-heading Chinese cabbage under adversity still less than the study of other model plants,especially the identification and response mechanism of transcription factor of non-heading Chinese cabbage under stresses have not been widely studied.Therefore,research of identification and response mechanism of transcription factor under adversity is one of the important methods for the study of stress resistance of non-heading Chinese cabbage.This experiment mainly through the comparison of the Arabidopsis CAMTA transcription factor genes and non-heading Chinese cabbage genome data,found out the non-heading Chinese cabbage CAMTA transcription factor family.I studied the conserved domain,transcription factor family and the clustering analysis respectively,subcellular localization and three secondary structure of protein were prediction by protein sequences.At the same time,the non-heading Chinese cabbage was subjected to stress,changes in the expression of the BcCAMTA gene family was observed,and analyze the expression profiles of BcCAMTA genes in five different growth periods.The main results were as follows:1.8 BcCAMTA genes were identified from non-heading Chinese cabbage genome,which belongs to the CAMTA transcription factor family of non-heading Chinese cabbage.Through the analysis of CAMTA family protein sequences of Arabidopsis and non-heading Chinese cabbage,CAMTA transcription factor family in non-heading Chinese cabbage was named as BcCAMTA1-6,and the conservative organization domains were analyzed.The conserved CAMTA domains were located on BcCAMTA protein sequences.The CAMTA transcription factor families were found in Chinese cabbage,cabbage and rice,and the cluster analysis was done in these plants.The prediction of subcellular localization and three structure of protein of BcCAMTA transcription factors family were finished through the websites of bioinformatics.2.Plants were exposed to cold stress,drought stress,heavy metal stress and nitric stress treatments.Then the expression of BcCAMTAs were measured,which were treated on 2 h,4 h,12 h and 24 h,to study the response of BcCAMTA transcription factor family under stress treatments.Among the findings,under cold stress treatment,the expression of amount of BcCAMTAs increased significantly,but in the drought,heavy metals and nitrate treatments,the expression quantity does not increase,on the contrary,its expression was inhibited,much lower than that of the control expression.It's different with the expression of CAMTA in other plants under adversity and the reason remains to explore.Among them,the expression of BcCAMTA genes under nitrate treatment was lower than expression of the control,but according to the results that,after 48 h,its expression increased significantly.Expression profile analysis of BcCAMTAs shown that the gene expression level of BcCAMTA3.1 and BcCAMTA5 in non-heading Chinese cabbage in growth period were significantly higher than other genes.Thus,BcCAMTA3.1 and BcCAMTA5 genes have a certain effect on development and growth of non-heading Chinese cabbage,but the specific mechanism remains to be further studied.3.Non-heading Chinese cabbage can be divided into five varieties,respectively.Respectively from the five variants to choose a variety of tests,to study the expression of BcCAMTA3.1 and BcCAMTA5 and physiological response of five varieties of non-heading Chinese cabbage under NaCl stress treatment.The results showed that BcCAMTA3.1 and BcCAMTA5 in response to NaCl stress,and the measurement results of physiological indexes show that the five varieties of non-heading Chinese cabbage resistance to NaCl stress generated.