| In dogs, mammary gland tumors are the most common, accounting for about 40-50% of all tumors in bitches. Approximately 50% canine mammary tumors are malignant, which severe threat the dog’s health. Canine mammary tumors are very similar to those in humans, which makes it a good model for comparative studies in terms of pathogenesis and classification. In this study, mammary tumors cases in bithces were collected and recorded in Nanjing. Breed, age, sex, fertility, tumor size and tumor location of these cases were systemic analyzed.Pathology of tumor tissue samples were observed and classified according to the standard. Meanwhile, tissues from 23 canine mammary tumors and 2 normal mammary glands were analysed for the expression of Tenascin-C protein and c-myc, c-fos and c-jun mRNA by S ABC immunohistochemistry and quantitative real time PCR. The aims of this study were to provide the reference data for pathology and diagnosis of canine mammary tumors.1. In the present study, the mammary gland tumor cases mainly affected hybrid canines, Pekingese dogs and Shih Tzu. The age of tumorous canines was mostly range 6 to 14 years old, and the average age was about 10±2.74 years old.95.7% cases affected bitchs without ovarian hysterectomy. Tumors were mainly located on the mammary glands of caudal abdomen and groin.2. The cases consisted of 7 benign tumors (7/23,30.44%),13 malignant tumors (13/23,56.52%) and 3 hyperplasia (3/23,13.04%). The benign tumors cases included 3 benign mixed tumor cases (42.85%),2 intraductal papillary adenoma cases (28.57%),1 fibroadenoma case (14.29%) and 1 complex adenomas case (14.29%). And for malignant tumors cases, there were 5 carcinoma-anaplastic cases (38.46%),3 carcinosarcoma cases (23.08%),2 carcinoma-cribriform cases (15.38%),2 carcinoma-solid cases (15.38%) and 1 carcinoma arising in a mixed tumor case (7.69%). All the hyperplasia cases were lobular ductal hyperplasia.3. The expression of Tenascin-C protein was investigated immunohistochemiclly in samples of normal canine mammary tissue (n=2), benign (n=13) and malignant (n=7) mammary tumours, hyperplasia (n=3). Tenascin-C protein was found in 3/7 benign tumors and 6/13 malignant tumors tissues, but no significant expression was observed in normal and hyperplasia mammary glands.4. The mRNA expression of c-fos, c-myc, c-jun were 14.67±8.39,4.14±2.09 and 2.94±2.37 in mammary malignant tumors,1.52±1.09,1.03±1.32 and 0.48±0.61 in benign tumors. Compared with normal mammary and benign mammary tumors, the mRNA overexpression of c-fos, c-myc and c-fos was observed in mammary malignant tumors tissues. There was no markedly difference between benign and normal tissues for the expression of these genes.In summary, mammary gland tumors mainly affected old bithes without ovariohysterectomy in these spontaneous cases. Carcinoma-anaplastic tumors predominantly occured in mammary malignant tumors. Benign mixed tumors were the most common type in benign tumors. Tenascin-C protein and mRNA of c-myc, c-jun and c-fos were over expressed in canine malignant tumors. These genes expression could be recommended as markers for differential diagnosis of this type of tumors. |
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