The Detection Of Plasmid-Mediated 16Sr RNA Methylase Genes In Escherichia Coli Isolates From Livestock Animals In Sichuan Province

With the scale and intensive animal farming industry, variety of stress factors were increased, which resulted in morbidity and mortality of infectious diseases remains high. Aminoglycosides as the control of major infectious diseases of livestock drugs, the drugs bacteria have developed resistance. Recently, studies show that plasmid-mediated 16S rRNA methylase to protect the bacterial 30S ribosomel6S rRNA,which can not be combined with the drug, including arbekacin mediate high levels of resistance to aminoglycosides including drugs. E. coli as an important habitat animal intestinal bacteria and pathogens, bearing a variety of drug selection pressure, forming a reservoir of multiple resistance genes, so its resistance testing, can understand the prevalence of resistance law, predicted the development trend of drug resistance, providing a scientific basis for risk assessment of antimicrobial drug use, and clinical rational use of antimicrobial drugs.Using the Kirby-Bauer method and the PCR-sequencing method to detect the (437 symbiotic microbes a and 82 clinical strains) 519 Escherichia coli which isolated from animal-derived drug sensitivity of 14 kinds of antibiotics, and some strains plasmid-mediated 16S rRNA methylase gene in this study. Susceptibility results showed that 437 symbiotic microbes to 14 antimicrobial agents showed different resistance, in which the three kinds of aminoglycoside resistance is low (resistance rate of 2.98%～30.9%); the rest of the 11 kinds of non-aminoglycosides showed varying levels of resistance, which is not the Meropenem resistance (resistance rate of 0.46%), ampicillin (69.79%), cotrimoxazole severe (68.72%); 71.12% isolates were multi resistant, resistant strains of 3 to 5 percentage than the highest of (31.58%), drug-resistant type of wide spectrum, there are 108 kinds of resistance spectrum type.82 clinical strains to 14 antimicrobial agents showed different resistance, in which the kanamycin, gentamicin and amikacin three kinds of aminoglycoside resistance is higher, respectively,100% and 89.02 and 32.93; the rest of the 11 kinds of non-resistance to aminoglycosides showed different levels, which in addition to the Meropenem no resistance (resistance rate of 3.66%), and the remaining resistance rates as high as 79.27%; 100% were multi-drug resistant strains resistance, resistant strains of 10 to 13 than among the highest percentage (84.14%), there are 23 kinds of resistance spectrum type, strengths resistance spectrum type AMP/A/C/CFZ/CTR /CTX/CN/CHL/DOX/NAL/CIP/SXT/KAN.134 aminoglycoside-resistant symbiotic microbes and 82 clinical isolates were 16S rRNA methylase gene (armA, rmtA, rmtB, rmtC, rmtD, rmtE, npmA) of PCR and PCR-sequencing test. PCR results showed that 134 symbiotic strains, five carry rmtB gene (detection rate of 3.73%), not yet detected armA, rmtA, rmtC, rmtD, rmtE, npmA; 82 clinical isolates of bacteria,10 genes containing rmtB (12.20%), one containing rmtA (1.22%), and co-exist with rmtB and rmtA.134 aminoglycoside-resistant commensal bacteria and 82 clinical isolates were 16S rRNA methylase gene (armA, rmtA, rmtB, rmtC, rmtD, rmtE, npmA) of PCR and PCR-sequencing test. PCR results showed that 134 symbiotic strains, five carry rmtB gene (detection rate of 3.73%), not yet detected armA, rmtA, rmtC, rmtD, rmtE, npmA; 82 clinical isolates of bacteria,10 genes containing rmtB (12.20%), one containing rmtA (1.22%), and co-exist with rmtB and rmtA.134 aminoglycoside-resistant commensal bacteria and 82 clinical isolates were 16S rRNA methylase gene (armA, rmtA, rmtB, rmtC, rmtD, rmtE, npmA) of PCR and PCR-sequencing test. PCR results showed that 134 symbiotic strains, five carry rmtB gene (detection rate of 3.73%), not yet detected armA, rmtA, rmtC, rmtD, rmtE, npmA; 82 clinical isolates of bacteria,10 genes containing rmtB (12.20%), one containing rmtA (1.22%), and co-exist with rmtB and rmtA.