| In order to explore gene expression differences and function in Adelphocoris suturalis Jakovlev development, we constructed the cDNA library of egg, nymph-1, nymph-3, nymph-5, adult of Adelphocoris suturalis respectively and carried the transcriptome sequencing using the HiseqTM2000 platform, and assembled the sequencing reads into unigene using the mix together, and conducted annotation of GO functional classification and KEGG pathway analysis to the unigene.Besides, we found out the unigene of differently expressed related to during the different stadium of Adelphocoris suturalis under the transcriptome comparison during the different stadium of Adelphocoris suturalis, following the example of serine protein gene, verify the difference of gene function,which contributes to understanding the function of the other differential genes in the future. The main conclusions of the study are as follows:(1)There are two kinds scent glands in Adelphocoris suturalis Jakovlev, one is named abdominal scent glands,the other is metathoracic scent glands. They have different position in different development. In the nymph stage, the openings of scent gland are located in the abdominal back plane that between the abdomen fourth abdominal segment and the fifth abdominal segment; in the adult stage, the openings of scent glands are located in the metathorax.(2)There had 20,842 of 57,480 unigene obtained annotation information after function annotation of gene using the blast tools, and 16,872 of which is annotated to Nr, 16,014 unigene with GO function classification information, 12,119 to the Swiss-Prot, and 5,495 unigene with annotation information in KEGG pathway, but more than 63.75% unigene had no function information. These detailed function and classification information provide essential information for the study of Life activities and gene regulation which is Adelphocoris suturalis Jakovlev taken part in.(3)We extracted 4,460 DEGs between the nymph-1 and egg sample, and 4,785 DEGs between the nymph-3 and egg sample, and 4,595 DEGs between the nymph-5 and egg sample, and 4,787 DEGs between the adult and egg sample, and 2,830 DEGs between the nymph-1 and adult sample, and 2,797 DEGs between the nymph-3 and adult sample, and 2,890 DEGs between the nymph-5 and adult sample, and 200 DEGs between the nymph-1 and nymph-3 sample, and 796 DEGs between the nymph-5 and nymph-3 sample, and 900 DEGs between the nymph-5 and nymph-1 sample, using random sampling hypothesis testing of the value of FPKM. These unigene provide important information for the identification of Unigene which is related to growth and development.(4)The results of GO function classification and enrichment analysis of above DEGs showed that Between nymph-1 and eggs, the annotation of gene function, mainly involved in single organism metabolic process, extra cellular region, oxidoreductase activity etc. Between nymph-3 and eggs, the annotation of gene function, mainly involved in carbohydrate metabolic process, nuceus, structural constituent of cuticle etc. Between nymph-5 and eggs, the annotation of gene function, mainly involved cell, organelle, binding etc. Between adult and eggs, the annotation of gene function, mainly involved proteolysis, respiratory chain complex IV, catalytic activity etc. Between nymph-1and adult, the annotation of gene function, mainly involved oxidation reduction process, luextra cellar region, hydrolase activity etc. Between nymph-3 and adult, the annotation of gene function, mainly involved hydrolase activity, luextracellar region, hydrolase activity etc. Between nymph-5 and adult, the annotation of gene function, mainly involved hydrolase activity, cell-cell adhesion, tubulin complex etc. Between nymph-1 and nymph-3, the annotation of gene function, mainly involved structural constituent of cuticle, ploysaccharide metabolic process, structural constituent of cuticle etc. Between nymph-5 and nymph-3, the annotation of gene function, mainly involved chitin metabolic process, structural constituent of cuticle,structural etc. Between nymph-1 and nymph-5, the annotation of gene function, mainly involved luextracellar region,structural constituent of cuticle, hydrolase activity etc.(5)At the same time, the results of annotation and enrichment analysis of DEGs in KEGG pathway showed that there have DEGs in 156 of 257 KEGG pathway between the nymph-1and eggs, 11 of which is DEGs significantly enriched pathways, there have DEGs in 159 of 257 KEGG pathway between the nymph-3 and eggs, 13 of which is DEGs significantly enriched pathways, there have DEGs in 153 of 257 KEGG pathway between the nymph-5 and eggs, 10 of which is DEGs significantly enriched pathways, there have DEGs in 154 of 257 KEGG pathway between the adult and eggs, 15 of which is DEGs significantly enriched pathways, there have DEGs in 137 of 257 KEGG pathway between the nymph-1 and adult, 7 of which is DEGs significantly enriched pathways, there have DEGs in 138 of 257 KEGG pathway between the nym-ph-3 and adult, 6 of which is DEGs significantly enriched pathways, there have DEGs in 142 of 257 KEGG pathway between the nymph-5 and adult, 3 of which is DEGs significa-ntly enrich-ed pathways, there have DEGs in 31 of 257 KEGG pathway between the nymph-1 and nymph-3,2 of which is DEGs significantly enriched pathways, there have DEGs in 89 of 257 KEGG pathway between the nymph-5 and nymph-3, 4 of which is DEGs significantly enriched pathways, there have DEGs in 104 of 257 KEGG pathway between the nymph-5 and nymph-1, 3 of which is DEGs significantly enriched pathways.(6)According to the results of the transcriptome sequencing stitching design primers, RACE were used to amplify partial cDNA fragments and full cDNA, one serine protein gene from Adelphocoris suturalis was identified, designated ASJSP. The full-length cDNAs of ASJSP contain 885 bp open-reading frames(ORF) which encode a putative 295 amino acid protein. We speculated that the cloning of protein sequence belongs to the family of serine protein. |
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