| This study used five conventional wheat varieties(xinong 889, xiaoyan 22, xinong 2611, aikang 58, zhoumai 18) and one hybridstrain(xiza 13) were used in part one. The standard germination test, accelerated aging test, conductivity determination, seedling growth test, optical density(OD) determination and simulate the field emergence rate six methods were conducted to determine the viability of wheat seed, seed germination potential, germination rate, dry weight, vigor index, root length, seedling length, conductivity, light density and other indicators were measured to analyze the correlation between the vigor indexes and the seedling emergence rate. Found out effective indexs for reflect the seed viability. Determination of seed vigor of constructed by 175 wheat varieties(hybridstrain) in association mapping populations and 56 pairs of SSR markers were used for scan the whole genome and genotype phenotype correlation analysis. Three wheat varieties in low vigor and three copies of high activity wheat varieties chosen as parent materials at the same time, according to NCII incomplete dialed cross design configuration of the nine hybrids, combining ability and genetic parameters were analyzed.1. Simulated field seedling emergence rate and seedling dry weight, standard vigor index, shoot length, accelerated aging germination power, conductivity, relative conductivity, the optical density indicators are good methods to evaluate the seed vigor of wheat and field survival ability.2. 56 pairs of SSR markers were used to scanned 175 wheat materials and 253 alleles were detected, single labeled to detect allelic variation biggest is 11, least is 2, average 4.5, genetic diversity ranged from 0.139 to 0.848 with average is 0.554; polymorphism information content was between 0.132 and 0.829, average 0.503. Indicated the experiment materials used in this study, the genetic diversity was rich available for the correlation analysis.3. The tested materials were divided into 3 subgroups according to group structure analysis and UPGMA cluster analysis based on genetic distance. The 1 groups had the highest vigor, the 2 the second, and the 3 lowest in groups, two methods had the same consistency.4. 20 loci with 13 markers associated with phenotypic traits were detected, and the rate of phenotypic variation was between 6.66% and 35.21%. Four markers associated with the absolute aging absolute elecrical conductivity, there have four markers associated with the conductivity, and aging electrical conductivity associated 3, aging germination rate and relative electrical conductivity are associated with 2 markers, and germination rate, aging germination potential, absolute electrica conductivity, aging relative electrica conductivity associated markers was one, respectively is Xwms293, Xwms295, Xbarc78 and Xwms102. Marker Xwms295 was associated with 5 vigor traits.5. The heritability of vigor index and CAT were lower. The main performance of the additive genetic effects of genetic activity characters were germination potential, germination index, the main performance of the non additive gene effects of the genetic characteristics were conductivity and relative conductivity; the effects of additive and non additive gene effects on the traits were germination rate, SOD and POD.6. In the six parent materials tested, the M1, M3, M4, M5 general combining ability(GCA) was better, and were chosen as good parent material. M2×M4, M3×M6, M1×M4, M2×M5 and M3×M5 combination, which showed a good special combining ability(SCA). |
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