| Seeds have extremely important biological and production practical significancein in agricultural production can genetic.By artificial accelerated aging to mimic natural aging test method on wheat seed vitality of QTL mapping can be for the future work provide theoretical basis for breeding high vigor seeds.In this study,160 introgression lines(IL)constructed from Lumai 14/jing 411 were used as materials.Determination of the germination rate,the seed germination potential,germination index,seedling length,vigor index,Simple vigor index and length of root of standard(E1),aging 24 hours(E24),48 hour(E48),60 hours(E60)and 72 hours(E72)5 environments were measured.The effect of aging treatment on seed vigor and the correlation between the vigor indexes were analyzed,and the QTLs were mapped.The purpose of this study was to explore the genetic mechanism of wheat seed vigor,and to provide basis for molecular marker assisted selection of high vigor seed.The main findings were as follows:1、The critical change point of the seed vigor-related traits were after aging treatment 24h-48h.And there were significant or extremely significant correlations between the seven traits of the IL group in five treatment.2、IL its seed vigor traits phenotypic variation in each groups were biased the recipient parent lumai 14,which was consistent with the characteristics of the introgression lines.Most of the Phenotypic value of seed vigor related traits were in normal distribution,which could be used to study the localization of wheat seed vigor traits.3、We identified seven QTLs of seed vigor related traits of wheat,such as GP,GR,GI and so on,under five different treatments.A total of QTLs for 17 wheat seed vigor traits were found and other than GP were detected and distributed in 1A,1D,2A,3B,4D,5A,5B,7B 8 chromosomes,including 16 additive QTL synergistic alleles from the donor parent jing 411.A total of epistatic QTL255 pairs were detected and distributed on all chromosomes except 6D.The contribution rate of epistatic QTL to phenotypic variation was 7.8178%-35.0429%,which was much higher than that of additive QTL(5.3687%-11.9731%).The epistatic QTLs were significantly larger than the additive QTLs in the detected number,the distribution and the contribution to phenotypic variation,indicating that the wheat seed vigor was more affected by the epistatic effect.4、In this study,four additive QTL hot spots were detected,including QVi-1D、QSvi-1D、QRI-1D which near Xgdm 126;QSL-5A、QVI-5A、QSVI-5A which were near Xgwm205;QSl-4D、QVi-4D、QSVi-4D、QRl-4D which were near gwm624;QGe-1A、QGi-1A、QVi-1A which were near Xwmc24.Epistatic QTL hotspots had 45,and the effect of the locus on the phenotypic epistatic effect was significant.The marker which near Xwmc616 were detected control of all seven traits(GP,GE,GI,SL,VI,SVI,RL)epistatic QTL.The Epistatic QTLs near the locus of markers Xwmc24,Xbarc96 and Xgwm371 affected 6 of the seven traits of seed vigor;The Epistatic QTLs in the vicinity of the markers Xgwm337,Xgwm205,Xgdm 126,Xwmc740,Xgwm205 and Xgwm359 affect five of the seven traits of seed vigor;The epistatic QTLs at markers near Xwmc754,Xwmc667,Xwmc407,Xwmc262,Xwmc177,Xwmc517,Xwmc331,Xwmc201 affected 3-4 of the seven seed vigor traits.These hotspots can provide a reference for the future analysis of wheat seed vigor traits5、We detected same wheat seed vigor traits under different aging time,the site of QGe-1A were detected at the same time in E48,E60 and E72,QVi-1A was detected under conditions of E48 and E72.These QTL loci synergistic alleles were derived from the donor parent,jing 411,indicated that jing 411 was influnced the seed vigor.These results have a reference for the future selection of high-viable seeds. |
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