Study On Screening Of Clubroot Resistance Germplasm Resources And Hybyid Combinations Of Chinese Cabbage

This experiment through to highly resistant to clubroot of Chinese Cabbage Variety ’Afro Asian No.4’,anti clubroot Chinese Cabbage Variety ’CR-D25’, root to clubroot of Chinese Cabbage Variety ’CR yellow core’, a sense of clubroot of Chinese cabbage cultivar ’Golden Delicious’ as test material, respectively, discussed the different inoculation concentration and different inoculation methods, seedling substrate and Plasmodiophora brassicae liquid different mix ratio and Taibai Mountain Area clubroot occurrence area of different sources of soil on the effect of soil disease. meanwhile,The seedling stage using artificial inoculation method and field using serious areas of natural induction method for Northwest A & F University College of horticulture,cruciferous vegetables breeding team selection by 78 Chinese cabbage different product lines and hybrid combinations were identification disease resistance. And excellent resistance to clubroot of hybrid combinations identified by biological characteristics research. The results of the study are as follows: 1.Effects of different seeding concentration on the susceptibility of the hostwhen the spore concentration reached 2x108 / ml, incidence and disease index in the middle level, the repeat relatively stable and smaller differences or no differences, and in the other two concentration the overlap between the unstable and had significant difference. So it can be concluded that the best inoculation concentration is 2x108 /ml. 2.The influence of different inoculation methods on the incidenceFive kinds of inoculation methods on clubroot have effect, but inoculation effect difference greatly. By compared to better injection method and dipping root method, the general effect of other three inoculation methods. 3.Effects of different proportions of different proportions of seedling substrate and root swelling pathogen liquid on the incidence of the diseaseIn the four ratio, with the increase of the substrate in the ratio, the decrease of the spore concentration, the incidence of the disease is getting lower and lower, and the disease index is getting smaller and smaller. experiment conclusion: When the matrix and bacteria ratio of 1:1, the incidence and disease index is the highest. 4. Effects of different sources of soil on the incidenceThree kinds of soil pH and concentration of spores in the measurement results: the pH of collected In the acquisition of south slope and kawamichi soil samples from Taibai Mountain area are the same, the soil samples in the northern slope are acidic; concentration trend from high to low is: on the southern slope of the soil sample, kawamichi soil sample, on the northern slope of the soil sample collection. The incidence from heavy to light: the southern slope of the soil samples, the northern slope of soil samples, kawamichi soil samples. 5.The use of artificial inoculation in seedling stage and field induced by two kinds of identification methods of natural resistance to a comprehensive appraisalThrough the outcome of clubroot resistance identification of the 78 samples of different Chinese cabbage varieties and hybrid combinations by injection method at seedling stage of artificial inoculation and field endemic areas by natural two identification methods of view, seedling by artificial inoculation and serious areas of high resistance and high sense of identification of the material of high accuracy using natural induced by the two different identification methods, consistent with the rate of 100%; But the accuracy of disease resistance, disease resistance and susceptible material identification is relatively low, with only 20.00%- 59.26%; At the same time, the screened high anti cruciferous clubroot ’DS11-61’, DS18-4 ’and’ DS6-6 ’3 Chinese Cabbage Germplasm Resources and’ 14 CR spring 6 ’and’ 15 Cr Nongda 1’2 copies of excellent hybrid combination. ’14CR spring 6’ showed early maturity, high low-temperature bolting, high yield, disease resistance and high quality; ’15Cr Nongda 1’ disease resistance, high quality, high yield and stable performance, strong adaptability resistance low temperature bolting.