Behavors Of Matrine In Soil And Water Environments

Matrine has high fungicidal and insecticidal activity, and it has been current researched focus on the development of botanical pesticide. In this research, environmental behavior of matrine, such as its absorption, mobility, hydrolysis, photolysis, soil degradation, were studied using the ‘Experimental Guideline for Environmental Safety Evaluation of Chemical Pesticide’, a method for residue determination of matrine developed. The main results and conclusions are as follows:A rapid, accuracy sensitive method for residue determination of matrine in soil and water was developed using UPLC.The line equation was y = 12195x-10443, with the linearity ranging from 0.01 to 100 mg/L(R2 = 0.9987). The method had been validated by spiking different concentrations of matrine to soil and water samples.The results showed that the mean recoveries for soil were from 74.7% to 88.8%, spiked at 1.0 to 10.0mg/kg(RSD < 7.58%), Mean recoveries for water were from 94.2% to 107.5%(RSD <5.58%).The LOD were 0.1 mg/kg, 0.01 mg/kg, for soil and water samples, respectively. It indicated that the developed method could be applied for residue determination and environmental behavior of matrine.The result of oscillation balance experiment showed that the Kd values in Henan fluvo-aquic soil, Guanzhong lou soil and Northern east black soil were 20.63, 21.32 and30.09, which indicated it was medium difficult to absorb by those three soils. Matrine was relatively difficult to absorb in Jiangxi red soil, with the Kd values of 7.31. There was a positive correlation between absorption coefficient of matrine in four different soils and clay content(P = 0.049), while OM, p H and the soil property CEC had little effect on Kd.The results of soil thin layer analysis showed that the mobility of matrine in different soils was easy to move(Rf > 0.90), but the movement patterns of matrine in different soil layers were different. With the soil extension(6-12 cm), matrine in Henan fluvo-aquic soil,Guanzhong Lou soil and Northern east black soil gradually reduced and matrine in Northern east black soil decreased most, while the content of matrine in Jiangxi red soil increases gradually. The mobility of matrine in Jiangxi red soil was the highest, followed by two in Henan fluvo-aquic soil, Guanzhong Lou soil, while Northern east black soil was theweakest.Degradation experiment of matrine in soil showed that the half-life of matrine in sterilized soil ranged from 4.1 to 9.8 d with the temperature was 25 o C, while the half-life in the non-sterilized soils varied from 11.6 to 13.7 d, much longer compared with that in sterilized soil, suggesting that it was easy to degrade. It can be concluded that matrine is generally safe to soil environment.Indoor simulation experiment was conducted to study the hydrolysis dynamics of matrine at different p H values, temperatures and degradation characteristics and influence factors of matrine in natural water, the results showed that: matrine in the sterilized buffer hydrolysis degraded slowly. Matrine in natural waters degraded rapidly and the half-life varied from 6.26 to 12.81 d, its degradation rates were different in different natural waters:pond water > river water > rain water > sea water > lake water > tap water; degradation rate slowed down with the increase of initial concentration, half-life of matrine was positively correlated with its initial concentrationbut with half-life less than 1 month, so matrine belongs to degradation. Through sterilized and non-sterilized experiments, indicating that microorganism was an important factor affecting the degradation of matrine in water(P =0.006).The photolytic characteristics of matrine in different aquatic solutions and its influential factors, including the initial mass concentrations, light sources, p H values,degradation products and organic solutions in laboratory conditions. The results demonstrated that: the photolytic rate decreased with the increasing initial concentration of matrine in pure water at a range from 1 mg/L to 20 mg/L, the half-life of matrine ranged from 13.30 to 27.62 min. The photolytic rates of matrine under different light sources showed the following sequence: 1000 W high-pressure mercury lamp >1000 W xenon lamp> natural light, the half-life of matrine were 10.55, 22.07, 42.01 min, respectively. The photolysis half-life of matrine decreased with the p H of the buffer solution at a range from 4up to 9, the half-life of matrine ranged from 19.75 to 52.12 min. The photolytic rates of matrine under different natural waters showed the following sequence: apond water > river water > lake water > sea water > rain water > tap water > ultrapure water, the half-life of matrine in different natural waters in 0.5 h..In summary, soils had medium adsorbability to matrine and matrine was easy to migrate in these soils, but mainly concentrated in the surface soil, and susceptible to microbial degradation, not easy to remain. matrine was easy to be degraded in natural water,degradation for microbial degradation. The photolysis half-life of matrine in waterenvironment in 1 hour. Therefore, matrine wass more safe to soil and water environment.