Monitoring Of Cydia Pomonella And Clone, Polyclonal Antibody Preparation Of CpomSNMP2

The codling moth, Cydia pomonella(L.), as a worldwide distribution fruit pest, is a primary quarantine pest in China. The major hosts of the codling moth are apple, pear, peach, walnut and many more pome fruits and walnuts. The larva of codling moth jeopardizes fruit of plants, to resulting the quality and financial loss. The codling moth mainly distributes in the fruit production area of Xinjiang and Gansu provinces. Recently, the trend of the insect is from west to the east of China. In our study, the sex pheromone traps placed in Lanzhou and Tianshui of Gansu Province were used for monitoring of codling moth. Meanwhile, we also cloned a relative gene. Our study not only plays a vital role in monitoring the spread of codling moth trends but also provide a basis of prevention and control.The monitoring time of this study is from April to October in 2015 and monitoring locations are three orchards in the city of Lanzhou and Tianshui(Gansu Province). Based on the statistics of the number of insect population in the sex pheromone traps, the result showed found that the codling moth is not found in village of Huaniu in Tianshui,but appared in the village of Xiuchuanxin and Qingbaishi in Lanzhou. Accordingly, the colding moth have not spread to Tianshui, but the increasing population indicated the surveillance is still seriously. Sensory neuron membrane proteins(SNMPs) are critical peripheral olfactory proteins and highly promote the sensitivity of pheromone detection. In this study, we cloned a SNMPs transcript(CpomSNMP2, GenBank accession number: KU302714) from the antennae of the codling moth Cydia pomonella based on the transcriptome. The open reading frame(ORF) is 1575 bp in length and encodes a protein with 524 amino acid residues. CpomSNMP2 contains two putative transmembrane domains together with an extra-cellular loop. Phylogenetic analysis showed that SNMPs can be divided into SNMP1 and SNMP2 subgroup and CpomSNMP2 was assigned into the SNMP2 subfamily. Based on prokaryotic expression, we gained the recombinant proteins. The purified protein was further injected into the New Zealand white rabbits and gained the polyclonal antibody. The enzyme-linked immunosorbent assay(ELISA) results showed that polyclonal antibody prepared was satisfied to the tissue localization experiments.