| Using natural enemy to control pest is the key function of biological control. The generalist predaceous stinkbug Arma chinensis(Fallou) is a kind of valuable natural enemy to control pest. They can prey on Lepidoptera, Hymenoptera, Hemiptera and Coleoptera. They are considered as valuable natural enemies because of their wide distribution, strong adaptability and predation capability in the field. However, in the application process, due to the lack of appropriate prey as food, the stinkbug don’t have been put into use successfully. So the main purpose of this article is to find appropriate insect as prey to propagate A. chinensis. To investigate the suitability of larvae of Mythimna separata(Walker) and Corcyra cephalonica(Stainton), and the pupa of Antheraea pernyi(Guerin-Meneville) as alternative host of A. chinensis, we tested development time, body size(weight and length), fecundity, oogenesis rate, longevity and other life history parameters of A. chinensis that respectively reared on three prey species; Nutrition analysis of 3 prey species were also studied. At last, through metabolomics technology, we find metabolite content and metabolic pathway in A. chinensis. The results suggested that M. separata could be the substitutive prey for mass rearing A. chinensis in biological control programs.All these three prey species can feed the stinkbug successfully. The body size and sexual ratio were not affected by the food treatments. There was no significant difference in development time, survival rate, fecundity, and egg hatch rate between M. separata and A. pernyi. However, the stinkbug reared on C. cephalonica had longer development time, lower survival rate and fecundity compared with those reared on M. separata and A. pernyi. The developmental duration from egg to adult was 29.88 days, which is significantly longer than others reared on M. separata and A. pernyi that the duration was 25.57 days and 26.15 days respectively. The fecundity of one generation has an amount of 231.44, which is significantly lower than others reared on M. separata and A. pernyi that the amount was 360 and 441 respectively. The adult obtained rate was 23.78%, significantly lower than others reared on M. separata and A. pernyi that the rate was 60% and 60.56% respectively. However, the longevity of female adult of stinkbug reared on C. cephalonica was longer than those reared on M. separata and A. pernyi.Based on the nutrition component of 3 prey species, the results show that the hightest level of component in larvae of M. separata, C. cephalonica and pupa of A. pernyi are respectively glucose, crude fat and protein. Among the three kinds of prey, the highest levels of two kinds of amino acids are the glutamic acid and aspartic acid. The stinkbug feed with C. cephalonica have one nutrient content significantly lower than that of the other. It is the level of α-Linolenic acid.Through metabolomics variance analysis on the stinkbug feeding on three prey species, the results are as followed. When the stinkbug was feeding on larvae of M. separata and C. cephalonica, a plurality of Allantoin、AMP、Glucose、Proline、Citrulline、Choline were significantly different. The stinkbug fed with this two kinds of preys have different metabolic pathways. When the stinkbug was feeding on larvae of M. separata and A. pernyi, a plurality of Allantoin、Citrulline、sn-glycero-3-phosphocholine、trehalose、Glucose、Histidine were significantly different. The stinkbug fed with this two kinds of preys have similar metabolic pathways. When the stinkbug was feeding on larvae of C. cephalonica and A. pernyi, a plurality of Allantoin、Betaine、Citrulline、Proline、Histidine were significantly different. The stinkbug fed with this two kinds of preys have different metabolic pathways. |
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