Effects Of XFM And Its Specificity Antagonist On The AMPK In Rats’ Brain

In order to investigate the effects of XFM and the antagonist for XFM on AMPK in different encephalic region of rats, clearing the change trend of AMPKα1 mRNA 、AMPKα2 m RNA、AMPK protein and p-AMPK protein during anesthesia and wakeing up, and discuss the anesthesia and analeptic mechanisms, all above can provide a reference to better guide the clinical medication in the future.90 SD rats were divided randomly into three groups: XFM Anaesthesia Group(M group, n=30), Antagonist Group(W group, n=30), and XFM and Antagonists interactive Group(MW group, n=30). Animals in M group were randomly divided into five subgroups: C1 group(rats injected 0.9%NaCl with the same dose of M group), M1 group(rats received XFM intraperitoneally and then waiting for 10 minute), M2 group(rats received XFM intraperitoneally and then waiting for 20 minute), M3 group(rats receive d XFM intraperitoneally and then waiting for 40 minute), M4 group(rats received XFM intraperitoneally and then waiting for 1 hour); Animals in W group were randomly divided into five subgroups: C2 group(rats injected 0.9%NaCl with the same dose of W group), W1 group(rats received the antagonist for XFM intraperitoneally and then waiting for 10 minute), W2 group(rats received the antagonist for XFM intraperitoneally and then waiting for 20 minute), W3 group(rats received the antagonist for XFM intraperitoneally and then waiting for 40 minute), W4 group(rats received the antagonist for XFM intraperitoneally and then waiting for 1 hour); Animals in MW group were randomly divided into five subgroups: C3 group(rats injected 0.9%NaCl with the same dose of MW group), MW1 group(rats received the antagonist for XFM after injected XFM intraperitoneally to anaesthetize 10 minute, then waiting for 10 minute), MW2 group(rats received the antagonist for XFM after injected XFM intraperitoneally to anaesthetize 10 minute, then waiting for 20 minute), MW3 group(rats received the antagonist for XFM after injected XFM intraperitoneally to anaesthetize 10 minute, then waiting for 40 minute), MW4 group(rats received the antagonist for XFM after injected XFM intraperitoneally to anaesthetize 10 minute, then waiting for 1 hour). The brain tissues were taken when reaching each time point, separating cerebral cortex, cerebellum, thalamus, brainstem and hippocampus. The transcription of AMPKα1 mRNA and AMPKα2 mRNA in different encephalic region was detected with real time PCR. The expression of AMPK protein and p-AMPK protein in different encephalic region was detected with Western Blot.The results show as follows:1.After the administration of XFM, the transcription of AMPKα1 mRNA and AMPKα2 m RNA in five encephalic region were increased significantly; the expression of p-AMPK protein in cerebellum, hippocampus, thalamus and brainstem were increased significantly. The change of protein and m RNA were at equal pace.2.Injection of the antagonist for XFM alone could significantly decline the transcription of AMPKα2 m RNA in five encephalic region; the transcription of AMPKα1 m RNA in cerebral cortex and brainstem were declined significantly, while the transcription of AMPK α1 m RNA in the thalamus were increased significantly; the expression of p-AMPK protein in cerebellum, hippocampus, and brainstem were declined significantly.3.Using the specificity antagonist for XFM to wake the rats anaesthetized with XFM may cause the significant decline of the transcription of AMPKα1 mRNA in cerebral cortex and brainstem, while the transcription of AMPKα1 m RNA in the cerebellum, hippocampus and thalamus were increased significantly; the transcription of AMPKα2 mRNA in cerebellum, thalamus and brainstem were declined significantly, while the transcription of AMPK α2 mRNA in the cerebellum and hippocampus were increased significantly; the expression of p-AMPK protein in cerebellum, hippocampus and brainstem were declined significantly.In conclusion: There were some activation effects of XFM on the transcription of AMPK α2 mRNA and the expression of p-AMPK protein. The effects of antagonist for XFM were opposite with the XFM. But the antagonist can not completely antagonistic the activation effect of XFM on the transcription of AMPKα2 mRNA and the expression of p-AMPK protein in the process of waking up. It prompted that the study on the mechanism of general anaesthesia were com plex and multidigit.