| Flowering Chinese cabbage(Brassica campestris L. ssp. Chinensis var. Utilissen. et Lee) is a variant of Brassica and produced in China’s Yangtze River region, its edible organs is tender flower stalk. In this study, two flowering chinese cabbage sterile line as female, 17 male inbred by incomplete diallel hybridization formulated 34 crosses, Agronomic and quality traits, yield traits of the F1 offspring and parents were analyzed the heterosis and combining ability to breeding the excellent parent and high quality hybrids. This study analyzed the relation of parents genetic differences and heterosis Using SSR markers for exploring yield and quality of cabbage hybrids in line with consumer demand people. Maintainer lines 15, 16 and their corresponding male sterile line 15 A, 16 A are compared from flower organ morphology, pollen viability, molecular identification, growth potential and pod shape. The research results saw blow:1. Agronomic traits, yield traits of 34 crosses heterosis are showing positive average mid-parent heterosis, in which the weight of main stalk, number of lateral flower stalk, weight of pre plant three traits are the highest heterosis. But quality traits hardly have negative advantage heterosis.2. The parents were screened by analysis of combination ability, the CMS line 15 A which number of lateral flower stalk and weight of lateral flower stalk have a ppositive effect with force, it can improve the yield of the lateral stalk as parental. The CMS line 16 A have posive GCA in main flower of stalk, it can as parent to significantly improve the main stalk market ability. The male C23, Y3 can be used as high yield breeding parents; B26, B22 can be used as improving the nutritional value of parent; By SCA effect analysis of hybrid combinations, none of the various traits of hybrids are excellent, relatively speaking, 15 × B26, 15 × C23, 15 × Y3, 15 × B30, 16 × C23, 16 × B26, 16 × B43, 16 ×Y3 these eight hybrids SCA is relatively high and have evidentally good traits.3. Analysis of the traits associated with yield analysis by using Grey Incidence Analysis, The range of correlation degree is 0.7908-0.8777, which most closely related traits are lateral stalk weight, relevance reaches 0.8777, followed atalk-leaf ratio, the number of lateral stalk, so in the breeding process, we should pay attention to lateral stalk weight, number of lateral stalk and stalk- leaf ratio selection for high yield breeding.4. The 19 materials were divided into four groups at the place that genetic similarity coefficient was 3.80 through SSR markers. Group I including C23, B31, Japanese caixin, B26, C23 and Japanese caixin are blong flowering chinese cabbage, B26 and B31 with small narrow and green leaves, it may be from the hybridization crossed by cabbage and cabbage sprouts; Group II including B43, B30, B37, B39, 15 A, except 15 A very early 30-35 days, the other four growth period are 45 days or so; Group III including B12, B07, B10, B27, B11 of the five materials belong late parents, growth period are more than 60 days, B12 extremely late bud about 100 days; Group IV including B42, Y3, B04, 16 A, B22, which leaves are too large, oval. B04, Y3 blade folding like cabbage, 16 A, B04, B22 leaves yellow-green.5. Through the CMS line and its maintainer line for analysis, the flower organ and phenophase of the maintainer is similar to its corresponding CMS, the maintainer has normal anther with pollen viability and it is capable of pollination, seed filling and seed rate. The CMS line with anther deformity and no pollen viability. Using specific primers Orf138 for molecular identification, the CMS line can spread out around 300 bp bands, the results showed that the CMS line contains radish 0gura sterile cytoplasm. |
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