Preparation Of Fibrauretin Oral Liquid

Palmatine to Menispermaceae dried plant cane in yellow vine alkaloid extracted, as a plant extract is " Republicof China Veterinary Pharmacopoeia" collection contains.Preliminary Research and Development of Fibrauretine Oral Liquid.Fibrauretine is extracted from plants of Chinese herbal medicine and it has wide pharmacologic action of anti-virus, anti-gram positive bacterium, anti-gram-negative bacterium, anti-fungus and anti-parasite.1. Purpose:Developed a non-resistance, low toxicity of pure Chinese herbal medicine treatment of E. coli diarrhea disease. Let the plant antibiotics-Palmatine better clinical application.2. Materials and methods:Optimizing of fibrauretine extraction process. Preparation and quality evaluation of fibrauretine oral liquid.Acute toxicity test of fibrauretine oral liquid. In this study, security preliminary evaluation of fibrauretine oral liquid was made using acute toxicity test in laboratory rat,which clarifid toxicity extent of fibrauretine oral liquid. Bacteriostatic test in vitro of fibrauretine oral liquid.pharmacologic action of fibrauretine solution and oral liquid was studied using drug sensitive test. Palmatine oral clinical trials: the screened clinically positive for E. coli disease naturally infected 120 pigs were randomly divided into 2 groups of 60. A group Palmatine oral treatment experimental group, another group andrographolide injection in the treatment group.3. Result:By means of single factor experiment original selection and orthogonal design optimization, extraction ratio of fibrauretine was 1.05% and purity of it was 94.5% on condition that concentration of acid water was 2%, PH value 10 and agent ratio 1:6. To improve product quality Palmatine oralcontrollability, to establish the content of the active ingredient palmatine index of Palmatine oral HPLC detection method. In the change by speeding, low temperature, high temperature, light and other factors, the use of high performance liquid chromatography to detect its content, its content did not change significantly. The test result showed that the maximum tolerance dose of fibrauretine oral liquid was 395mg/kg. Fibrauretine was non-toxic medicine and its LD50 could not be determined. Therefore, fibrauretine was non-toxic medicine which accorded with security standard of clinical administration. It showed that fibrauretine oral liquid restrained staphylococcus aureus and escherichia coli differently. Its minimal inhibitory concentration to staphylococcus aureus was 662.5μg/ml and to escherichia coli was 312μg/ml. clinical trial results show that the experimental group palmatine Oral E. coli was 88.35% cure rate in the control group Andrographis Injection E. coli cure rate is 70%.4.In conclusion:Pure Chinese herbal medicine Palmatine oral drug is safe and capable of E. coli diarrhea disease has a good cure effect, it could be extended.