Identification And Analysis Salt Stress Response MiRNA Gene And Chloroplast RNA Editing Sites Of Wild Emmer

Wild emmer(Triticum dicoccoides) as the wild ancestor of tetraploid and hexaploid cultivated wheat. Due to wild emmer wheat have many important agronomic characteristics, which considered to be important wheat breeding germplasm bank.MicroRNAs(miRNA) are small non-coding single-stranded RNA and widely present in vivo.Which can combination to the target genes through complementary base pairing to regulation of plant physiology and biochemistry action. RNA editing, through a series of base insertion at the mRNA level, deleted or modified bases and other genetic information to make RNA maturation process, is one of the most important post-transcriptional regulation methods of gene expression of higher plants chloroplast genome.In this study, the plant material RNA samples were sent to the company to operated high-throughput sequencing. What is more,predicted and identified the chloroplast genes of RNA editing sites in wild emmer wheat through bioinformatics and RT-PCR binding methods.The results showed that:1.190 precursor conserved miRNAs, 161 mature conserved miRNAs,223 precursors novel miRNAs, 140 mature novel miRNAs were obtained.2.QRT-PCR showed that the expression level of seven mi RNA, including mi RNA166 b, mi RNA171 a, mi RNA393 a, miRNA N25, miRNA N38, miRNA N41, miRNA N92, have significant difference when subjected to salt stress. It indicating that these miRNAs may be stress response gene of wild emmer wheat under salt stress.3.Bioinformatics tools were used to predicted chloroplast genome sequence of wild emmer wheat. The results showed that 35 editing sites of 15 genes almost C to U conversion. 9 sites namely ndhB was the largest number of editing sites.4.Random selected editing site of five genes were verification. The results identified that a total of 18 editing sites was C to U. Secondary structure and transmembrane domain of the encoded protein was predicted. Found that secondary structure of all genes was occurs changing, while transmembrane domain of ndhB was occurs changing.5.Finally, RNA editing sites of chloroplast genome in wild emmer compared other seven gramineous species, it was found 17 editing sites were conserved between these species.Compared to other species, wild emmer wheat,common wheat and coarse goat grass have more similar composition of editing site.In this study, miRNA and RNA editing in chloroplast genes of wild emmer wheat under salt stress have been studied, qRT-PCR and RT-PCR were used to verified the accuracy of results. This study provides relevant research about mechanisms for wild emmer wheat response to salt stress.