Degradation Of Cellulose To Produce Methane Flora Study And Application In Food Residues

This research is based on the natural flora from different habitats conditions, used daily biogas production, total biogas production and biogas methane content as screening index, anaerobic enrichment in nutrient solution and the giant JunCao as matrix, after enrichment and equipping, an efficient degradation of giant JunCao compound strains producing biogas. During the fermentation temperature of 37℃, inoculation amount for 5%, medium for fermentation under the condition of 100 mL, after 40 days can produce 1219 mL biogas production, biogas methane content can reach 62%. Compared with single source strains, equipping the compound strains producing gas start faster, acidification phase reduced 3-5 days, methanation phase ahead 2-3 days,have a longer time stable gas phase, can make whole fermentation cycle time 5-8 days ahead of time, gas production increased by 25%-50%.Different fermentation stage’DNA were extracted with CTAB-NaCl respectively, using bacteria and archaea 16S rDNA primer amplification its conservative sequence, connect the purpose fragment to pMD18-T carrier, into a competent E.coli, pick scriptures colony PCR positive clone child after validation testing. Results show that 90 bacterial clones, among them 25 belongs to Clostridium sp.,21 belong to Tre-ponema sp.,15 belongs to Eubacterium sp., belong to 8 Bacteriodetes sp.,2 belong to Prevotella sp.,19 are Uncultured, accounted for 27.8%,23.3%,16.7%,8.9%,2.2%, 8.9% respectively; 84 archaea clones, among them38 belong to Methanofollis sp.,22 belong to Methanosarcina sp.,7 belong to Methanobacterium sp.,2 belong to Methanocorpusculum sp.,15 are Uncultured, accounted for 45.2%,26.2%,8.3%, 2.4%,17.9% respectively, showed that the biogas fermentation bacteria flora is a complex system, biogas production is the result of multiple microbial interaction.The number of bacteria and methane bacteria for different stages were studied through SYBR Green RT-PCR. Results show that the early stage of high yield gas bacteria fermentation, to the 10st day, the amount of bacteria is 2.0×109/mL, the amount of methane bacteria is 7.7×103/mL; the middle stage of fermentation, to the 20st day, the amount of bacteria is 5.2×107/mL, the amount of methane bacteria is 1.7 ×104/mL; the later stage of fermentation, to the 30st day, the amount of bacteria is 7.7 ×107/mL, the amount of methane bacteria is 1.1×103mL. Community quantity and gas production have obvious corresponding relation in the process of fermentation.Biogas fermentation by the equipped composite strains for three types of food residues, bagasse, waste mushroom drum and bamboo shoots. Three kinds of raw materials all showed good effect on gas production, more than 8 L gas production af-ter 30 days fermentation. Compared with total biogas production, bamboo shoots is the highest, waste mushroom drum is lower, bagasse is the last. Compared with biogas methane content, waste mushroom drum is the highest, bamboo shoots is lower, ba-gasse has a great change.