| Sipunculus nudus Linnaeus belongs to Sipunculidea and Sipunculus, which is a kind of unique rare economic marine species in the beibu gulf China. The effect of respiratory metabolism for the different environment factors (temperature, alinity and feed concentration) and different body weight of S. nudus Linnaeus were studied using experimental ecology methods in this paper. Through this study, not only can reveal the impact of environmental factors on physiological ecology mechanisms, and can rich in biological research content of S. nudus Linnaeus. Consequently promote the healthy development of aquaculture of S. nudus Linnaeus to provide necessary theoretical basis. The results were as follows:1. The effects of temperature (20℃、23℃、26℃、29℃、32℃) and body weight S-(1.59 ± 0.36 g)、M-(6.53 ± 0.83 g) and L-(9.36 ± 1.01 g) on oxygen consumption rate (OR) and ammonia excretion rate (AR) of S. nudus Linnaeus were studied in the laboratory. The results indicated that temperature had significant effect on OR and AR (P< 0.05), and their interaction also had significant influence on OR and AR (P< 0.01).Both OR and AR decreased at first and then increased with temperature increment, and reached its valley at 26 ℃. The relationship between OR or AR and body weight can be represented all by the quadratic equation:Y=aX2—bX+c, where a values of OR or AR range is 2.138-2.534 and 0.009～0.012, b values range is 8.699～10.556 and 0.455～0.593, c values range is 5.908～9.734 and 4.369～5.275. The O/N value ranged from 1.45 to 2.21. The study showed that S. nudus Linnaeus has a strong ability to adapt to the change in temperature, which can adapt to the low temperature and high temperature by adjusting physiological metabolism.2. The effects of salinity (15.20、25、30、35) and body weight S-(2.11 ± 0.60 g), M-(6.31 ± 0.85 g) and L-(8.79 ± 1.40 g) on oxygen consumption rate (OR) and ammonia excretion rate (AR) of S. nudus Linnaeus. The results indicated that salinity had significant effect on OR and AR (P< 0.05), and their interaction also had significant influence on OR and AR (P< 0.01). Both OR and AR increased at first and then decreased with the salinity increment, and reached its peak at salinity 25. The relationship between OR or AR and salinity can be represented by the quadratic equation Y=-aX2+bX-c, where a values of OR or AR range is 0.030-0.044 and 0.009-0.012, b values range is 1.525～2.252 and 0.455～0.593, c alues range is 11.824～20.132 and 4.369～5.275. The O/N value ranged from 4.06 to 14.28. The study showed that S. nudus Linnaeus can adapt to the low salinity and high salinity environment by adjusting physiological metabolism, and the salinity 25 might be the optimum for the growth.3. The effects of feed concentration (0.5 g/L、1.0 g/L、1.5g/L、2.0 g/L、2.5 g/L) and body weight S-(1.58±0.48 g), M-(4.90±1.06 g) and L-(7.96±0.94 g) on oxygen consumption rate (OR) and ammonia excretion rate (AR) of S. nudus Linnaeus. The results indicated that feed concentration had significant effect on OR and AR (P< 0.05), and their interaction had significant influence on OR and AR (P< 0.01). Both OR and AR decreased with the feed concentration increment. The relationship between OR or AR and feed concentration can be represented by the linear equation Y=-aX+b, where a values of OR or AR range is 2.961～3.735 and 0.328～0.541, b values range is 18.009～24.093 and 2.353～3.520. The O/N value ranged from 3.04 to 11.34. The study showed that S. nudus Linnaeus can adapt to the low concentration and high concentration by adjusting physiological metabolism. |
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