Studies On Flight Capacity And Genetic Diversity Based On ISSR Markers Of Exorista Civilis Rondani

Exorista civilis, belongs to the Diptera:Tachinidae, was the main parasitic natural enemy insects, its main host insects have the Dendrolimusspp, Agrotis ypsilon, Helicoverpa armigera, Argyrogramma agnata etc, especially to the Ostrinia nubilalis, Loxostege sticticalis, Mythimna separata have a better control effect, which was one of the important advantages of parasitic natural enemy of its larvae. And it was the main parasitic natural enemy insects for insect pests of the farming, the forestry, the fruit and the vegetable. In recent years, there was a gradually attention by people, due to it has a prevention and cure function for a variety of grass pests.Due to the effect of Exorista civilis’s pest control was found in recent years, the study of Exorista civilis concentrated in the macroscopic aspects mainly, such as the biological characteristics, host selectivity and artificial feeding. In order to control the grassland pests by making full use of the natural enemy insects of Exorista civilis. Systematic study the flight ability under the conditions of different temperature and humidity, the effect of temperature and humidity interaction on the flight capacity of Exorista civilis, the accumulation of energy material in different days of Exorista civilis, the dynamic energy consumption of flight in the Exorista civilis and different geographic population genetic diversity of ISSR analysis in this paper, the results were as follows:1. Effects of the different temperatures and humidities on the flight capacity of 20 generations Exorista civilisStatistical analysis showed that suitable for flight of 20 generations E. civilis was 21 ℃ and 40% RH. Within a certain temperature range, the flight ability of 20 generations E. civilis was enhanced with increasing temperature, when the temperature was 21 ℃, flight ability reaches a maximum, then its flight capacity decreases with increasing temperature. Within the scope of certain humidity, the flight ability of 20 generations E. civilis was fall with the humidity rises, the ability to fly was best when the relative humidity was 40%.2. The influence on flight capacity of the different generations Exorista civilis in temperature and humidity interactionThe suitable temperature range:21℃～25℃, humidity range:40%～60%, for flight of the 20 generations E. civilis, but suitable temperature range:20 ℃～24℃, humidity range:40%～60%, for flight of the 2 generations E. civilis.3. The 20 generations Exorista civilis in different days accumulation of energy substancesThe research results show that the age of 5, the 20 generations E. civilis of female reached the peak in glycogen and glyceride, respectively was:114.3517 mmol/L and 0.7537 mmol/L. The E. civilis of male at age of 7, reached the peak in glycogen and glyceride, respectively was:111.6680 mmol/L and 0.7214 mmol/L.4. The 5 days of the 20 generations Exorista civilis dynamic energy consumption of flightThe change in different fly time of the energy levels for 20 generations E. civilis at age of 5, with the extension of flight time the glycogen content decreased significantly after 60 minutes in flight, and there was a significant difference in other flight time; Glyceride content decreased significantly after 120 minutes in flight, and the consumption amount of glycogen was two orders of magnitude higher than the number of glyceride. The main energy material was the glycogen at the fly time of the 20 generations E. civilis, and the glyceride was second.5. Genetic diversity and genetic differentiation of Exorista civilis from different geographical populations based on ISSR markerThe results showed that eleven primers with stable polymorphic were sieved from primers of ISSR and a total of 166 clear ISSR bands with high repeatable were amplified from the 80 individuals, on average, each primer amplified segments were 15.0909, and these were all the polymorphic bands, Polymorphism Information Content (PIC) of per locus was 0.8441～0.8653; the Shannon’s Information Index (1) and the Nei’s Gene Diversities Index (H) were 0.1240-0.3455 and 0.0841-0.2285, respectively; Coefficient of Gene Differentiation (Gst) was 28.78%, the index of Gene Flow (Nm) an average of 1.5702, that is, genetic variability mainly exists in individual within population, and moderate gene flow among the different popilations; E. civilis individuals from eight different habitats natural populations were divided into four groups and between the genetic differentiation of interspecific and geographical distance show a positive correlation.