Immune Antibody And Etiology Detection Of Peste Des Petits Ruminants In Inner Mongolia Region

Peste des Petits Ruminants (PPR),which mainly infect goats,sheep and other wild small ruminants, is an acute contagious desdly disease with the clinical characteristic of fever, stomatitis, diarrhea and pneumonia. It is listed by OIE as an animal epidemic disease with legal weight to be reported annually. It is categorized into the first class animal disease in China.One of the most important prevention methods for PPR is vaccination in China. Compulsory vaccination has been carried out for two years in Inner Mongolia since occurrence of PPR in Bayannaoer City in February 2014. To understand and master the effect of vaccination of PPR and the situation of wild virus infection, the immune antibody of PPR and specific nucleic acid of PPRV were detected by ELISA and real-time florescence RT-PCR respectively in 12 league cities and 2 country planning solo cities. The project would provide some practical reference for the prevention and control departments to draw up scientific and reasonable methods of prevention and control to PPR in the future.From Nov.2014 to Dec.2015,3749 serum samples was detected by ELISA and 3721 swab samples of eyes conjunctiva was detected by real-time fluorescence RT-PCR. The serum samples and the swab samples of eyes conjunctiva were collected from sheep and goats in different regions of Inner Mongolia. The results of immune antibody detection indicated that the PPR immune status of sheep and goats in Inner Mongolia region was averagely good and the antibody positive rates of each batch were up to 70%, which suggested that the vaccination efect of PPR was well and sheep and goats had basic ability to resist the infection of PPRV For the past two years in Inner Mongolia.However, the immune antibody positive rate of PPR was less than 70% in some league cities, which suggested there was a risk of PPR infection. The results of etiology detection of PPR indicated that specific nucleic acids of PPRV had not been detected in the 12 league cities and 2 country planning solo cities all, which suggested that there was no PPRV infection in sheep and goats or infection probability was very low.