Effect Of Bacillus Megatherium On The Production Performance、Nutrient Utilization And Intestinal Microflora Of Geese From 1 To 70 Days Of Age

This experiment was conducted to test the growth performance, nutrient utilization and intestinal micro flora of geese in response to dietary Bacillus Megaterium. Two hundred and sixty 1-day-old healthy male geese with similar body weight were randomly divided into four groups with five replicates in each group and 13 geese per replicate. The geese in control group (group A) were only fed with basal diet, and experimental groups (groups B,C and D) were fed with basal diet supplemented with 20,40,60 mg/kg Bacillus Megaterium, respectively. The experiment lasted for 70 days. The growth performance, slaughter performance, nutrient utilization, intestinal microflora etc were studied through animal feeding, metabolism trials and microorganism experiments treated by Bacillus Megaterium. The results were shown as follows:1. Growing performance:Compared with the control group,Bacillus Megaterium significantly stimulated the average daily gain of geese at the age of 1 to 28 days (P<0.05), The ADG of adding 60 mg/kg Bacillus Megaterium in the diets was higher than that of 20 mg/kg. Groups with 40 and 60 mg/kg Bacillus Megaterium had higher average daily feed intake than the control group from 29 to 70 days of age(.P<0.05).2. Slaughter performance:the gizzard index jejunum index,eviscerated rate and semi-eviscerated rate were were significantly higher in geese fed with 60 mg/kg Bacillus Megaterium higher than those in control group at the age of 70 days in the group(P<0.05).3. Nutrient utilization:Compared with the control group, the apparent metabolic rate of dry matter was improved significantly(P<0.05), the apparent metabolic rate of crude protein was improved(.P<0.1), the ammonia emission(P<0.05), uricase (P<0.05) and uric acid(P<0.01) were reduced significantly in geese fed with 60 mg/kg Bacillus Megaterium.The urea nitrogen in excrement and the NH3-N、 xanthine oxidase activity in blood of geese at the age of 28 days were reduced significantly as compared with the control group (P<0.05).The urea nitrogen in blood began to reduce significantly when the supplementation of Bacillus Megaterium reached upto 40 mg/kg (P<0.01)4. sIgA content:the sIgA levels in jejunum of geese at the age of 28 days were significant higher in the group fed with 60 mg/kg Bacillus Megaterium (P<0.05)5. Intestinal bacterial diversity:the quantity of microflora in different intestinal segment was less in the group with 60 mg/kg Bacillus Megaterium than other groups, and the microflora similarity degree decreased when Bacillus Megaterium increased from 20 to 60 mg/kg; BLAST analysis showed that,the similarity of 16S DNA between the dominant bacteria and the sequences in GENE BANK had a similarity is above 96%, and the uncultured bacterium clone might be some new genus or species of microbie. It deeded to be studied in the future.Above all, by refining the composition of intestinal microflora, Bacillus Megaterium could enhance the intestinal mucosal immune, as well as the Nutrient utilization, ultimately promote the growth of the geese with a dietary supplementation of over 40 mg/kg.