Effects Of Bacillus Amyloliquefaciens On Growth Performance,Immune Function,Antioxidant Capacity And Intestinal Microflora Of Piglets

The aim of this study was to investigate the effects of Bacillus amyloliquefaciens on growth performance,immunologic function,antioxidant capacity and intestinal microbial flora in piglets,and was to explore the application effects and feasibility of Bacillus amyloliquefaciens,replacing antibiotics,and provide reference frame of the reasonable utilization by determining the proper dosage preliminarily in piglets production.The first trial was conducted to study the effects of Bacillus amyloliquefaciens on growth performance,apparent digestibility and serum biochemical parameters in piglets.A total of 490 piglets(body weight 9-10 kg)were procured and randomly allocated into 7 groups with 8 replicates.Piglets were fed with basal diets(negative control group),basal diets supplemented with 0.01%?0.02%?0.04%?0.1%?0.2%Bacillus amyloliquefacicens(treatment groups),or basal diets supplemented with 0.0075%chlorotetracycline and 0.0020%flavomycoin(positive control group)for 35 days,respectively.The results showed as followed:In the present study,average daily gain(ADG)in the 0.02%Bacillus amyloliquefaciens group significantly increased 9.84%(P<0.05)as compared with the negative control group.ADG of experimental diets supplemented with 0.01%and 0.04%Bacillus amyloliquefaciens increased 6.65%and 5.05%(P>0.05)as compared with the blank control group,respectively.Dietary treatments had no effects(P>0.05)on apparent digestibility and serum biochemical parameters in piglets.The second trial was conducted to study the effects of Bacillus amyloliquefaciens on antioxidant capacity and immunologic function in piglets.The results showed as followed:Diets with 0.02%Bacillus amyloliquefaciens increased 19.58%(P<0.05),or 22.54%(P<0.05)in hepatic T-AOC as compared with the negative and positive control group,respectively.Diets with 0.02%Bacillus amyloliquefaciens had a tendency(P<0.1)to increase T-SOD enzyme activity as compared with the negative and positive control group.sIgA content of jejunum mucosa in 0.02%Bacillus amyloliquefaciens group increased 25.76%(P<0.05)compared to negative control group,and increased 21.08%(P<0.05)compared to positive control group in piglets.Piglets diets supplemented with 0.2%Bacillus amyloliquefaciens tended to increase serum lysozyme activity and IgG content(P<0.1)as compared with the negative control group and positive control group.The third trial was conducted to the effects of Bacillus amyloliquefaciens on intestinal microbial flora of alimentary canal in piglets.The results showed as followed:Dietary supplementation of Bacillus amyloliquefaciens had significantly influence(P<0.05)on the intestinal microbial composition in piglets.Dung Lactobacillus bacteria content in 0.02%Bacillus amyloliquefaciens supplementation group increased 42.83%(P=0.15)as compared with negative positive control group.LactoBacillus bacteria content in 0.2%Bacillus amyloliquefaciens supplementation group increased 92.04%(P<0.05)compared to negative control group.In summary,our results suggested that the dietary supplementation of 0.02%Bacillus amyloliquefaciens could improve growth performance,immunologic function,antioxidant ability and digestive tract micro-ecology in piglets.