| Maize heterosis utilization significantly increased yield, improve quality, has great economic value, is the main way of using maize breeding. Heterosis group,building the model is to reduce blindness equipping hybrid heterosis, improve breeding efficiency is one of the main means. Non-Reid group is the important germplasm groups of spring maize in north China area, will exert positive effects on maize breeding and production.Maize DH breeding technology fast cultivation fine inbred line department’s new breeding method.Because obtains the DH lines not yet to pass through the natural selection, conducts the heterosis research and the combining ability determination to it is extremely essential.Partial separation is refers to the offspring, genotype(tag) observation value with the theoretical value of Mendelian inheritance. Partial separation is considered to be an important power of crop evolution, such as DH population obvious separation occur, will severely limit its use value. Partial separation can’t use the traditional theory and method of genetic analysis, so separation study using molecular markers.This study based on Non-Reid, a group of backbone inbred lines equipping materials, using DH breeding technology for DH lines. By 20 DH lines as the female parent and Reid groups of inbred lines PH6 WC, zheng 58,DOM groups of inbred lines S122,chang 7-2, A87 as male parent, according to the NC Ⅱ genetic mating design, equipping 20×5 hybrid combinations, the hybrid F1 heterosis of yield and main agronomic traits, combining ability analysis,etc., for maize heterosis group and provide a new technical method to use heterosis pattern.SSR markers for the induced haploid separation study, verify whether the partial separation phenomenon is obvious.The results of the study are as follows.20 DH, five test piece together by 100 hybrid combinations of heterosis results show that the yield of F1 heterosis are widespread, there is biggish difference between different combinations of heterosis. DH lines 4H1-1, 4DH1-2, 4DH1-9, and 4DH1-14,respectively PH6 WC, Zheng 58 with the Reid Group, with DOM group of the S122,Chang 7-2 hybrid, F1 Heterosis in yield averages were higher;Yield per plant excessive advantage shows that 4DH1-14×Chang 7-2,4DH1-2×Chang7-2,4DH1-4×PH6WC,4DH1-3×PH6WC,4DH1-6×Zheng58,4DH1-5×Zheng58,4DH1-13×Zheng 58,4DH1-12×A87,for superior combination;4DH1-18×Zheng 58,4DH1-20×Chang 7-2,4DH1-19×A87 is a combination of weaker advantage;Heterosis for average and Heterosis of main agronomic indices analysis showed that Heterosis of yield and panicle weight of largest, stem diameter, ear diameter, number of rows per ear, ear axis diameter 4 characters of Heterosis is relatively low.General combining ability analysis showed that,4DH1-14,and 4DH1-1,and4DH1-9,and 4DH1-6,4DH1-10 yield GCA high;Combined with the General combining ability of yield traits, 4DH1-1,and 4DH1-5,4DH1-8,4DH1-9,4DH1-10,and 4DH1-14 integrated-like quality, staging a strong advantage of hybrids;Among them,the 4DH1-5,4DH1-14 group is the hybrid of both high-yield and lodging resistance and ease of harvest hybridized combination.Special combining ability effect analysis showed that different combination of the same traits of special combining ability effect very different, based on the analysis of various characters, this is the combination of high special combining ability effect in the experiment 4 DH1-4×PH6WC, 4 DH1-12×A87,4 DH1-2×chang 7-2, 4 DH1-13×zheng 58, 4 DH1-5×zheng 58, 4 DH1-3 ×PH6WC, 4 DH1-6× zheng 58.SSR markers isolated study showed that primer amplification band, the same as the parent band of haploid band from 1: 1 less, no significant differences, namely 80 strains parthenogenesis induced haploid partial separation phenomenon does not exist. |
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