| Bivalves in coastal sea areas ecosystem play an important role in energy flow and material cycle. The clam has rich nutrition, apprppriate price and very high economic value. In recent years, extreme climate happened frequently, this phenomenon bring a great changes to clam. We use clam as experimental material to study the clearance rate and respiratory metabolism of clam after temperature, air expourse and salinity stress. Through the study, we attempt to provide guidance and reference for the artificial breeding and long distance transportation of clam. 1、The temperature stress on clam clearance rate and metabolismThe experimental set four temperature group and measure the clearance rate, oxygen consumption rate and ammonia excretion rate at 6 time point. The result shows that the temperature stress has a significant influence on the clearance rate, oxygen consumption rate and ammonia excretion rate. 3 hours later after the clam into the different temperature water, the clearance rate and oxygen consumption rate have a peak variation and reached the maximum in 25°C and 30°C, and also the high temperature bring ammonia excretion rate rise. With the extension of time, the 20°C group’s clearance rate and ammonia excretion rate firstly decreases after the rise trend and get minimum value at 24 h, by the end of the experiment is still not back to the control level. At the same time, the ammonia excretion rate has no significant difference. The clearance rate of group 30°C lead to rise after the falling and at the peak of 12 h. Oxygen consumption rate is significantly higher than the control group, ammonia excretion rate continued to decline trend. The 35°C group of clam’s clearance rates and oxygen consumption rates continue to decline, and the clearance rate at 72 h is 0. The ammonia rate shows only at 3h. The results show that the temperature stress has a significant influence on clam physiological metabolism, and at the time extension, the influence is more significant. 2、The salinity stress on clam clearance rate and metabolismWe use static hydraulic test the clam clearance rate, oxygen consumption rate and ammonia excretion rate. The results show that the salinity stress has a significent influence on clam clearance rate and metabolism. In the process of the experiment we find that the lower the salinity the influence is deeper, and also the time under the stress the time to return to normal is longer. 3、The air expourse stress on clam clearance rate and metabolismUnder laboratory conditions, we study the water loss rate and the death rate in 6 days. And at the same time, we measure the clearance rate, oxygen consumption rate and ammonia excretion rate at 6 different time piont. The results show that in 24 h the water loss is 2.95%, and 10.30% in 72 h. The air expourse stress has significent influence on the clearcance rate, oxygen consumption rate and ammonia excretion rate. The clam of group 72 h opens shells at 24 h. With the time the clearance rate became increase to the controled group and the oxygen consumption rate has the peak variation. In conclusion, the air expourse stress has significent influence and at the time extension, the longer, the influence is deeper. 4、The air expourse stress on clam energy metabolism related enzymes.Using clam as experimental material, after difference air expourse time anatomical clams take its hepatopancreas and gills. Use kits to test pyruvate kinase(PK), lactic dehydrogenase(LDH) and Na+ K+-ATPase NKA to study the air expourse stress on clam energy metabolism related enzymes. The study shows that the air expourse stress has a significent influence on clam energy metabolism related enzymes, and also the different enzyme have different activity in different parts of clam. |
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