| Halymenia sinesis is subordinate to Halymenia, Halymeniaceae, Rhodophyta. Grateloupia C.Ag. is also belong to Halymenia, a genus where is most widely distribution. It has significant economic value,Such as, the rich amino acids and other nutrients to eat or make health products; the bright color and high agar content as industrial raw materials; the rich in alga polysaccharide, terpenoids, bromine phenolic compounds, alkaloids to anti-virus, anti-tumor, anti-blood clotting. In addition, due to the red color and beautiful shape, H. sinesis can be used as a kind of ornamental alga to artificial aquarium, oceanarium and so on.This experiment is mainly study the effects of different temperature and temperature fluctuation and light intensity and light quality on the growth,photosynthetic pigments, solub le protein, soluble sugar, anti-oxidative enzymes and membrane lipid peroxidation products of H. sinesis,which reveal the adaptation mechanism of H. sinesis on the climate change.Setting five different constant temperature(17, 21, 25, 29, 33℃), five temperature fluctuations(25±0、25±2、25±3、25±4、25±5℃), selectting four different wavelengths : red, blue, white and green, setting three different light intensity: high(3000lx), normal(1200Lx) and low( 500Lx). The results shows:(1) The growth of H. sinesis is significantly inhibited by the high and low temperature, and high temperature will cause cell death, which has greater impact on H. sinesis. At 33℃, H. sinesis gradually become soft, pale in color, white locally and fester, Chla and PE decreased more under high temperature than low temperature, At 25℃, Chla increased 56.71%, PE increased 30.72% at the end of fifteenth days. At 29℃, C hla and PE increased 37.09% and11.47 %. At 21℃, C hla and PE increased 24.18% and 13.68%. And the change of POD activity is not obvious at 17℃、21℃、25℃. At 29℃, the POD activity is 2.5 times that of the initial, At 33℃, it rised at first, and decline sharply later. The change of SOD and POD were similar, but the SOD activity was significantly greater than that of POD, which was 3.25 times higher than that of 25 ℃ after 15 days. At 25℃ or lower temperature, the MDA content changes little, at29℃, the MDA content is about 0.31 times of 25℃.At33℃, the MDA content higher than 25℃from the third to ninth day, then began to decline.(2)The proper temperature fluctuation is benefit to H. sinesis, and the Chla and PE are increased. In general the RGR:(25±2℃)>(25±3℃)>25℃>(25±4℃)>(25±5℃), with the increase of temperature range, the growth rate decreased obviously. Compared with(25℃), the PE change is stable early under(±4)and(±5℃) temperature range, fell 3.84% and 6.2% respectively later. C hla almost has no change. The C hla increased by 35.34% and 30.60% at the end under(±2℃)and(±3℃) temperature range.PE increased by 59.81% and 44.72%.At 25℃, the C hla increased by 17.19%, and the PE 27.06%.But the MDA content is stable under(±2℃)and(±3℃)temperature range, declining under(±5℃)temperature range, sharp declining under(±4℃) temperature range from the ninth day, dropped 12.51% than the initial value. The overall change of SOD and POD were also similar, the POD activity are about 3 times and 3.3 times that of constant temperature group under(±4)and(±5℃)temperature range, about 1.71 times under(±3℃)temperature range, and fluctuation on the whole process. And the SOD activity are very similar under(±2℃) and(±3℃) temperature range. But has different wave. The SOD increased 72.17% and 75.18%.than the beginning under(±4℃)and(±5℃)temperature range respectively.(3)The effect of light intensity and light quality on H. sinesis is complex. H. sinesis growth best under 1200 Lx with green light, among the three light intensities and four kinds of light, green light is the most conducive to the growth of H. sinesis, secondly is blue, white and red. High light intensity has larger impact on H. sinesis, red light appeared negative growth. Under 1200 Lx,C hla were increased by 35.08% and 37.68% with blue and green light. Under 3000 lx,Chla rising slowly at first, decrease slightly at twelfth days, but still higher than initially, increased by 8%.Red light, and white light were decreased by 58.07% and 12.5%, green light almost did not change eventually. Under 500 lx, red light decreased by 2.7%, green increased by 13.74%, blue light 11.83%, white light 4.34%. In addition to the 500 lx, the Car has been ris ing under blue light, rising firstly and decline finally under the green light. The changes trend is similar to Chla under the other light intensity. Under 1200 and 500 Lx, PE has been rising with green light, Under 3000 Lx, PE has no obvious change with green light, the other three kinds of light falling. Under 1200 and 500 Lx, the PC is 2.53 times and 1.84 times as much as the original with the green and blue light respectively, in addition to red light, in 500 Lx,the green, blue and white light were increased by: 23.2%, 15.03%, 13.03%. PC increased faster than PE in 500 Lx. SP increase faster in the blue and green light, SS increase faster in the red and green light, the change of the SP and SS are similar to the PE and PC. The change of C AT activity has litt le relationship with light quality, the influence of strong light on the POD, SOD, CAT and MDA value was greater than that of weak light, the effect of red light and strong light together on SOD is greater than POD. Under 3000 lx, SOD increased by 70.42%, POD is 56.99% with red light, and MDA is 4.03 times as much as 1200 Lx.To sum up, high temperature and low temperature, large temperature variations and different light quality and high intensity, all these affect the physiological and biochemical change of H. sinesis. Therefore, study the growth of specific conditions of H. sinesis clearly is helpful. |
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