| In aquaculture, the low feed utilization of fish decided to high pollution and the nature of the high consumption of nutrients. Biological flocculation Technology(Bio-flocs Technology, BFT), can be transform breeding solid particles which produced in the process of aquaculture into flocs bait that part of the breeding objects can ingesting again. BFT is applied to aquaculture in two ways, the first is forming flocs in the pond for aquaculture directly, called in situ-BFT; the second is reactor seprated from pond, and forming the bioflocs in sequencing batch reactors(SBR), and collecting the flocsforfeeding. In situ-BFT, however, might cause decrease of transparency and soluble oxygen. Its limitation in choosing of culture objects might also confine its advance. In this study, Biological flocculation Technology was used to culture biological flocs, the flocs are used to breed artemia. Basic biological parameters, nutrition and intestinal microorganisms of artemia were measured, to discuss the effect of bio- flocs as the arteima bait and to explore the further development and application of bio-flocs in aquaculture.The study mainly contains four parts below:1. Two different preservation methods of treatment on solid waste to bio-flocscomponent effectThis experiment were collected and stored after freeze-dried and fresh direct 4 ℃ preservation of the solid waste. Then these two different preservation methods solid waste was culture becomes biological flocs.Result shew that, freeze-dried to save solid waste, Free amino acids were 16 kinds of, and Docosahexaenoic acid(DHA) content was 0.15 mg/g. The preservation of fresh solid waste directly 4 ℃, Free amino acids were 23 kinds of, and Docosahexaenoic acid(DHA) content was 0.06 mg/g.2. Bait effect of use biological floc to fed artemiaIn this experiment, option flocs of fresh solid waste directly 4 ℃ as food for artemia, and chlorella as food a control. Result shew that, Group D( flocs feeding only) average body length of 8.53 ± 1.16 mm at 16 t h day, crude protein content of 52.84%, higher than the group A(chlorella feeding only), which average body length of 6.13±0.46 mm mm at 16 t h day, crude protein content of 45.97±0.53%. Showed biological floc can be used to develop artemia, promoting the growth of artemia, and achieve a higher nutrition.3. Preliminary study of using two different aquaculture waste culture bio- flocsIn this experiment, the eel and tilapia waste were used to culture biological floc, to discussion floc nutrient composition and microbial community structure characteristics. Result shew that, group of eel waste as a raw material for floc(A), crude protein was 35.59±0.23%, group of tilapia waste as a raw material for floc(B), crude protein was 29.29±34.95%. Shannon index of group A was 2.15 and group B was 2.37, Group B showed biological floc microbial diversity than group A. At the genus level in group A, total of 135 species of bacteria detection and abundance > 1% of the species has 11, group B detected 128 species, abundance species > 1% has 13.4. Study on using two kinds of biological floc that culture with differentaquaculture waste feeding arteimaIn this experiment, the two kinds of biological floc in test part 3 were used to breed arteima. Assay the biological characteristics, nutrition, and of intestinal microflora of the arteima, for evaluating the bait value of two kinds of biological floc as arteima food. Further explore the applicability and widely application of bioflocculation technology use in farming artemia. Result shew that, arteima of group A the crude protein was 70.25±0.92%, while group B was 65.60±0.95%. Artemia body EPA content of group A was 3.00 ± 0.46%, group B was 1.57±0.19%. Shannon index of group B the highest, 3.15 and Simpson index was the lowest, 0.13, arteima of group B showed biological floc microbial diversity than group A. At the genus level in group A, total of 175 species of bacteria detection and abundance > 1% of the species has 10, group B detected 182 species, abundance species > 1% has 11. |
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