Studies On The Bioactive Constituents Of Hypericum Ascyron L.

Hypericum ascyron L. which belongs to Guttiferae has been used widely for a long history in various regions of China. Its unique scientific value deserves to be explored. The topics in our paper mainly focused on chemical composition. We investigated that the compouds isolated from H. ascyron would have effects on Hep G2 cell line. Finally, we reviewed the protective of H. ascyron on D-galactosamine/LPS induced acute liver injury on mice.In this research, H. ascyron was extracted by 95% ethanol, and then was suspended in water and successively partitioned and extracted with petroleum ether, ethyl acetate and n-butanol. The active sites of the ethyl acetate was separated by different chromatographic methods, eight compounds were isolated from H. ascyron, their structures were elucidated by their physical and chemical properties and NMR techniques. Their structures were identified as quercetin (1), kaempferol (2),kaempferol-3-O-β-D-glucopyranoside (3), quercetin-3-O-β-D-glucopyranoside (4), hyperoside (5),4-Hydroxybenzoic acid (6), protocatechuic acid (7), a mixture of campesterol (a), stigmasterol (b), andβ-sitosterol (c) (8), compound (6) and (7) were isolated from the plant for the first time.H. ascyron that has a hepatotrotective effect was scavenged from 109 kinds of plants in Changbai mountain area by the method of tacrine-induced hepatotoxicity in Hep G2 cellsIn this part of the paper, we establish six teams of the experiment. They are the negative team with no drugs, the tacrine-induced team, and compound-treated team with concentration 20μM,40μM,80μM,160μM, resp. We screened out 2 compounds which have the highest hepatoprotective effects on tacrine-induced cytotoxicity in HepG2 cells from Hypericum ascyron L. by MTT method. The result of experiment is that the cell viability of quercetin EC50 was 239.3μM, and kaempferol EC50 was 84.3μM. Based on the appeal results, quercetin and kaempferol could reduce the cytotoxicity observably.