Association Of HSP70Gene Polymorphisms And Psychological Stress With Metabolic Syndrome

Objective1.To investigate an association between heat stress protein70genepolymorphism and metabolic syndrome and its related indicators among Hui and Hannationalities in Ningxia;2.To investigate the relationship of occupational psychological stressand metabolic syndrome and with HSP70gene polymorphism. To analysis the risk factors ofmetabolic syndrome among Hui and Han nationalities in Ningxia, providing a scientific basisfor the prevention of metabolic syndrome.Method According to a group case-control study design, workers of regionalorganizations and institutions aged20-65who received medical health checkup and endocrinepatients in Second Affiliated Hospital of Ningxia Medical College and Wu Zhong People,sHospital from March2010to October2011were enrolled in the study. According to theinclusion and exclusion criteria,303patients were identified as a case group,141men(46.53%) and162women (53.47%),153Hui (50.50%) and150Han (49.50%), mean age(50.56±5.91years), population from the same period in the Second Affiliated Hospital ofNingxia Medical College and Wu Zhong People,s Hospital out-patient clinics for regularhealth check-randomly selected303healthy people in the control group,141men (46.53%)and162women (53.47%),153Hui (50.50%) and150Han (49.50%), mean age (50.45±5.74years). Data were collected through questionnaire, physical examination and laboratoryexamination, and polymerase chain reaction-restrained fragment length polymorphismtechnique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms ofHSP70-1+190,HSP70-2+1267,and HSP70-hom+2437. Results1. There were no significant differences in the distribution of genotypes andallele frequencies of HSP70-1+190and HSP70-2+1267between the metabolic syndromegroup and control group(P>0.05), there were significant differences in the distribution ofgenotypes and allele frequencies of HSP70-hom+2437between the metabolic syndromegroup and control group(P<0.05or P<0.01); There were no significant differences in thedistribution of genotypes and allele frequencies of HSP70-1+190and HSP70-hom+2437between Hui and Han nationalities(P>0.05), there were significant differences in thedistribution of genotypes and allele frequencies of HSP70-2+1267between Hui and Hannationalities(P<0.05or P<0.01); There were no significant differences in the distribution ofgenotypes and allele frequencies of HSP70-1+190among abdominal obesity with metabolicsyndrome, non-abdominal obesity with metabolic syndrome group and control group(P>0.05);There were significant differences in the distribution of genotypes and allele frequencies ofHSP70-2+1267among abdominal obesity with metabolic syndrome, non-abdominal obesitywith metabolic syndrome group and control group(P<0.05), compared with metabolicsyndrome group, control and non-abdominal obesity group had significant differences in theA allele distribution of HSP70-2(χ~2=4.629，P<0.05，OR=1.106;χ~2=5.097，P<0.05，OR=1.242);There were significant differences in the distribution of genotypes and allele frequencies ofHSP70-hom+2437among abdominal obesity with metabolic syndrome, non-abdominalobesity with metabolic syndrome group and control group(P<0.05or P<0.01), compared withmetabolic syndrome group and control group had significant differences in the C alleledistribution of HSP70-hom+2437(χ~2=7.797，P<0.01，OR=1.381); By comparing the clinicaldata of different genotypes, we find that there were no significant differences in FPG, BMI,WHR, WC, SBP, DBP, TC, TG, HDL-C and LDL-C.of the genotype distribution ofHSP70-1(P>0.05);There were no significant differences in WC, SBP, DBP, TC, TG,HDL-C,BMI and WHR (P>0.05), but there were significant differences in FPG and LDL-Cof the genotype distribution of HSP70-2(P<0.05); There were no significant differences in WC, FPG,SBP, DBP, TC, TG, LDL-C,BMI and WHR (P>0.05), but there were significantdifferences in HDL-C of the genotype distribution of HSP70-hom(P<0.05).2.Occupationalstress test results showed that there were significant differences in the score of total stressfactors, workload, relationships, family/work balance and other sources (P<0.05), whilethere were no significant differences in the score of management roles, responsibilities,cognitive load and organizational climate between metabolic syndrome and control group(P>0.05); There were no significant differences in the score of work load, interpersonal,family/work balance, managerial role, work responsibilities, cognitive load, organizationalclimate and other sources between Hui and the Han population (P>0.05); There were nosignificant differences in genotype and allele frequencies of HSP70genes in the three stresslevels (P>0.05).3. The risk factors for metabolic syndrome included WC, WHR, DBP, FPG,TG, family history of diabetes, family history of hypertension, stress factors; the protectivefactors included standard of culture and HDL-C.Conclusion1. There were significant correlation among HSP70-hom＋2437andHSP70-2+1267with metabolic syndrome in Ningxia; HSP70-hom＋2437C allele carriersincrease the risk of metabolic syndrome in Han and Hui people;HSP70-2+1267A allelecarriers increase the risk of metabolic syndrome in Han people; HSP70-2+1267G allelecarriers increase the risk of metabolic syndrome in Hui people; There were significantcorrelation among A allele of HSP70-2+1267with abdominal obesity with metabolicsyndrome, it carriers increased risk of metabolic syndrome; There were significant correlationamong C allele of HSP70-hom＋2437with abdominal obesity with metabolic syndrome, itcarriers increased risk of metabolic syndrome; There were significant correlation amongHSP70-hom＋2437with dyslipidemia, it carriers increased risk of dyslipidemia.2. MS grouphad experienced more stress than control group, the source of stress factors mainly from workload, interpersonal relationships and family/work balance; Abdominal obesity ms group hadexperienced more pressure from family/work balance and work responsibilities than non-abdominal obesity MS group; Fasting blood glucose levels and high density lipoproteincholesterol is closely related with occupational stress; Hui and Han had the same level inoccupational stress; The levels of occupational stress were not related to the HSP70genepolymorphism.3. The risk factors for metabolic syndrome included WC, WHR, DBP, FPG,TG, family history of diabetes, family history of hypertension, stress factors; the protectivefactors included standard of culture and HDL-C.