Research Of Echinococcus Granulosus Recombinant14-3-3Induce The Host CD4~+T Cell’s Immune Regulation Mechanism

Objective we Proposed from the whole worm antigen of Echinococcus granulosus and recombinant antigen E.g14-3-3,to find the impact of changes dynamicly in the four sub-class of CD4+T cell-mediated host immune response, to explore rE.g14-3-3involved in Echinococcus granulosus infection of the host immune protective effect and related mechanisms. Methods132female BALB/c mice were randomly divided in rE.g.14-3-3immunized group, natural infection group and blank group, injected with rE.g.14-3-3and PBS Respectively, three times in all,at intervals of2weeks. Six weeks after the last immunization, in addition to the blank group, the remaining two groups of mice were injected with100uL Protoscoleces suspension intraperitoneally to attack infection. The mice week sacrificed after immunity and infection in different time, and spleen single cell suspension were prepared, Thl, Th2, Th17and Treg cells accounted for the proportion of CD4+T cells were measured dynamicly by flow cytometry at each time, then at last week, remaining mice in each group were sacrificed to observe the immune protection. Results The85.8%immune protection was obtained in rE.g.14-3-3immunized BALB/c mice; Flow cytometry results showed that:compared to blank group, Thl, Th2, Th17and Treg cells in the proportion of CD4+T lymphocytes in rE.g14-3-3immunized group showing an increasing trend each week after immunization(P<0.05). After challenging with Echinococcus granulosus infection, the proportion of Thl cells in rE.g14-3-3immunized mice increased at week2,6after infection, compared to the blank and natural infection control group (P<0.05); The ratio of Th2cells in rE.g14-3-3mice rised at week2,6compared to the blank group (P<0.01), and increased at week10,15,20after infection compared to the blank and natural infection control group (P <0.05); The ratio of Th17cells in rE.g14-3-3mice rised at week2,6after infection, compared to the blank and natural infection control group (P<0.05), then decreased at week15after infection compared with the blank and the natural infection control group (P<0.05); The proportion of Treg cells in rE.g14-3-3immunized mice were lower than natural infection group at each week after infection.(P<0.05). Conclusion r.Eg14-3-3has a ability to stimulate BALB/c mice get an effective cell immune response, After Protoscoleces attck, regulation response were inhibited in Treg cells in rE.g14-3-3immunized mice, Thl and Th17immune response dominanted in the early stages of infection, then Th2immune response play a mainly role in late stage of infection.