The Intervention Effect Of Sophoridine On Rat Liver Cirrhosis Formation And Lipopolysaccharide Level And Expression Of TLR4、NF-κB

Objective This study was to research the intervention effect of sophoridine on rat livercirrhosis formation and lipopolysaccharide level and the influence of expression of TLR4,NF-κB.Methods The rats were randomly divided into control group,liver cirrhosis modelgroup, the low,medium and high dose of sophoridine intervention groups,there are15rats ineach groups.The liver cirrhosis model group was made the model with carbon tetrachloridecomposite method. And the low,medium and high dose of sophoridine intervention groupswere injected Sophoridine3,6,10mg/kg on this basis.And observed the pathological changesof the liver and determinated the LPS levels. And detecting the expression of TLR4, NF-κB inliver tissue through Real-time qPCR.Results Pathological observation showed liver cirrhosis group have formed false lobe,while the three intervention groups have not formed false lobe.The population means of5groups’ plasma LPS concentrations were not equal and the differences between every twogroup were statistically significant （P<0.05）, The population means of5groups’ expressionof TLR4were not equal and the differences between every two group were statisticallysignificant（P<0.05）, The population means of5groups’ expression of NF-κB were notequal,the differences of expression of NF-κB in liver tissue between medium and high dose ofsophoridine intervention groups were not statistically significant（P=0.128>0.05）, pairwisecomparisons of the other groups were different（P<0.05）.The does of sophoridine and thelipopolysaccharide,TLR4, NF-κB was negatively correlated（r=-0.810、-0.824、-0.798， P<0.05）, the lipopolysaccharide in rat plasma and expression of TLR4, NF-κB was positivecorrelated（r=0.979、0.878，P<0.05）.Conclusion Sophoridine can play a role in intervene the formation of liver cirrhosisthrough anti-lipopolysaccharide. Different dose of sophoridine have different interventioneffects,there is a certain dose-response relationship. Sophoridine can delay the formation ofliver cirrhosis and reduce the lipopolysaccharide level through inhibit expression of LPS-TLR4-NF-κB signaling pathway.