The Expression And Significance Of LYVE-1、VEGF-C、HMGB1Protein In Human Cervical Squmous Cell Carcinoma

Object The text investigated the role and the significance of the LVD, VEGF-C andHMGB1in lymphatic metastasis of human CSCC (cervical squamous cell carcinoma).Method The expression of the LYVE-1(though the expression of LYVE-1to countLVD), VEGF-C and HMGB1were evaluated by immunohistochemical staining in15cases ofnormal cervical squamous epithelial tissue samples,15cases of CIN III (cervicalintraepithelial neoplasia) and76cases of CSCC. The relationships between their expressionand the clinical pathological parameters of CSCC were analyzed as well.Result （1）The counting of LVD：The counting of LVD in group with normal cervicalsquamous epithelial tissue was2.26±0.68, in group with CIN III was3.07±0.50，in groupwith CSCC was4.95±1.83. There was statistical difference（P＜0.01）.The expression ofLVD was closely correlated with clinical stage, lymphatic metastasis, tumor size andpathologic grading (P<0.05).（2）The expression of VEGF-C：VEGF-C mainly located incytoplasm of CIN III and CSCC. The positive rate of VEGF-C in group with normal cervicalsquamous epithelial tissue was0.00%, in group with CIN III was40.00%，in group withCSCC was67.11%. There was statistical differenc（eP＜0.01）.The expression of VEGF-C wasclosely correlated with clinical stage, lymphatic metastasis, tumor size and pathologic grading(P<0.05) in CSCC（.3）The expression of HMGB1：HMGB1mainly located in cytoblast of CINIII and CSCC. The positive rate of HMGB1in group with normal cervical squamous epithelialtissue was0.00%, in group with CIN III was26.67%,in group with CSCC was59.21%.Therewas statistical difference（P＜0.01）. The expression of HMGB1was closely correlated withclinical stage, lymphatic metastasis and tumor size (P<0.05) in CSCC, with no significant correlations with pathologic grading (P>0.05) in CSCC.（4）The relationship between theLVD、VEGF-C and HMGB1: Spearman correction analysis showed that there was a positivecorrelation between HMGB1and VEGF-C(r=0.781， P=0.000), there was a positivecorrelation between HMGB1and LVD(r=0.714, P=0.000), there was a positive correlationbetween VEGF-C and LVD(r=0.719，P=0.000).Conclusions1From normal cervical squamous epithelial tissue、CIN III to CSCC,LVD has increased and closely related with the lymphatic metastasis of CSCC. The growth oftumor lymphatic vessels played a critical role in lymphatic metastasis of CSCC.2VEGF-Cwas over expression in CSCC and closely related with the progression, invasion and lymphaticmetastasis of CSCC. VEGF-C can induce the growth of tumor lymphatic vessels and promotelymphatic metastasis of CSCC.3HMGB1was over expression in CSCC and closely relatedwith the initiation, progression, invasion and lymphatic metastasis of CSCC. HMGB1andVEGF-C had a synergistic effect in CSCC. HMGB1might induce the expression of VEGF-Cto promote lymphatic metastasis of CSCC.