| Objective: Normal cervix, carcinoma in situ and early cervical cancerantigen105(CD105), D2-40, vascular endothelial growth factor C (VEGF-C)were measured to understand the microvessel density (MVD) in varioustissues and lymphatic microvessel density (LMVD), whose results werecomprehensively analyzed according to the clinical stage and histologicaltype of patients to reveal the distribution changes of microvessel andlymphatic microvessel in the evolution of cervical cancer. Their clinicalpathological link and correlation between VEGF-C and MVD, LMVD valuewere also studied in order to help early diagnosis of cervical cancer lymphaticmetastasis and guide clinical therapy.Method：Detected10cases of normal cervical tissue,8cases of cervicalcarcinoma in situ,40cases of early cervical cancer， the expression of D2-40,VEGF-C, CD105in the tissue samples of by using Immunohistochemicalmethod. Chose three areas rich in blood and lymph vessels in each CD105,D2-40slice at low magnification (100x) to count MVD and LMVD at400xmagnification and observe the expression of VEGF-C in cervical tissue usinga semi-quantitative analysis.Result:1. The number of MVD labeled with CD105in normal cervical tissue,carcinoma in situ (0stage), clinical stage I cervical carcinoma, clinical stageII cervical carcinoma was:3.70±2.89,7.50±1.09,8.01±3.89,8.70±3.50 respectively. There existed significant differences between the four groups(p=0.019) by analyzing statistically with rank sum test.2. The number of MVD labeled with CD105in carcinoma in situ,adenocarcinoma, squamous cell carcinoma was:5.95±2.19,6.5±2.58,9.07±4.02respectively. There existed significant differences between the threegroups (p=0.048) by analyzing statistically with rank sum test.3. The number of LMVD labeled with D2-40in normal cervical tissue,carcinoma in situ (0stage), clinical stage I cervical carcinoma, clinical stageII cervical carcinoma was:5.39±2.73,7.59±1.93,7.07±2.43,5.55±2.45respectively. There were no significant differences between the four groups(P=0.649) by analyzing statistically with rank sum test.4. The number of LMVD labeled with D2-40in carcinoma in situ,adenocarcinoma, squamous cell carcinoma was:4.75±2.44,5.42±1.97,5.47±2.26respectively. There were no significant differences between the threegroups (p=0.602) by analyzing statistically with rank sum test.5. There was no expression of VEGF-C in normal cervical tissues. Thepositive expression rate of VEGF-C in cervical carcinoma in situ (0stage),clinical stage I cervical carcinoma and clinical stage II cervical carcinomawas25%,87.9%and90.9%respectively. There existed significant differencesbetween the three groups (P=0.000) by analyzing statistically with Fisher’sexact test.6. The positive expression rate of VEGF-C in cervical carcinoma in situ,adenocarcinoma and squamous cell carcinoma was62.5%,100%and93.8%respectively. There existed significant differences between the three groups(P=0.042) by analyzing statistically with Fisher’s exact test.7. There was a correlation between the expression of MVD marked with CD105and VEGF-C in cervical cancer cells. There was significantcorrelation between the groups (P=0.002) by analyzing statistically withSpearman rank correlation analysis method. The results suggested that MVDmay be helpful in the differential diagnosis of carcinoma in situ and earlyinvasive cancer.Conclusions：1. MVD marked with CD105increased gradually with the occurrenceand development of cervical carcinoma, indicating that CD105is related tothe occurrence and development of cervical carcinoma, which may be thetargeted therapeutic index of early cervical carcinoma.2. LMVD marked with D2-40increased gradually with the occurrenceand development of cervical carcinoma, which may serve as the predictorindex of early lymphatic invasion in cervical carcinoma.3. The expression of VEGF-C in cervical carcinoma was significantlyhigher than that in normal tissue. The expression of VEGF-C was related tothe pathological type and clinical stage; the later the stage, the higher theexpression, suggesting that VEGF-C may become an important index toreflect the biological behavior of cervical carcinoma.4. There is a correlation between the expression of CD105and VEGF-C.The combined detection of the two can help assess the prognosis of patients.5. There is lymphangiogenesis at early phase of cervical carcinoma. Thecombined detection of D2-40and VEGF-C is helpful for early diagnosis ofearly lymph node metastasis of cervical carcinoma and prognosis. |
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