| Clinical treatment for the bone defect, especially large segment bone defect arecertain difficulties, creating ideal conditions for bone formation in bone defect is hard toapproach, for the moment of this condition appears can lead to bone nonunion.thetreatment is a huge challengin especially when gunshot bone defect which is the specialtype of bone defect happens. Due to the lost of soft tissue and the existence of foreignbody which is not easy to remove can easily cause the bone defect site infection and be thebasis of osteomyelitis. in the war, the extremities are the most vulnerable body part to gethurt, the last century20’s, in all the famous battles, the probability of extremitymusculoskeletal injuries is60%[1], those damages often plays an important role to leadto the permanent disability [2], selecting the appropriate method to treat those wound isthe regular challenge to most clinicians, therefor the appearance of the bone tissueengineering treatment which studies the seed cells cell carrier, and osteogenic factoropened up a new path for the present situation and some of the occurrent studies havemade some encouraging results. Nevertheless, no artificial bone has achieved the idealcondition which can be comparable with the autologous transplant, so developing aautograft alike bone products is a serious challenge now, so the main purpose of this experient is:(1) to produce the sheep bone wound defect model under the same conditions,and using chemical method to create xenogenically and partially deproteinised bonebovine dice,using the bone combined with autologous red marrow engraft the defect partto observe the ability of formation of the bone tissue engineering bone and to certifywhether the defect can be fixed on the first stage and to provide a theoretical basis andnew methods for the early treatment of gunshot bone defect.;(2) within the given time toobserve the recanalization in trabecular bone marrow cavity and to compare withautogenous bone in order to understand whether this bone tissue engineering bone as theformer one. Materials and methods:(1) using a handsaw to cut the bovine bone in anappropriate size and managing the diced bone with chemical method;(2) the experimentalsheep were randomly divided into three groups, each of the sheep were made the rear righttibia bone defect in the same condition and in6hours, all of the defect were debridedand fixed by bone grafting and internal fixation, and then observe the changes ofexperimental sheep postoperative activities and life signs;(3)X-ray test was taken everymonth to observe the growth of the defect callus and to evaluate the osteogenesis ability ofgraft;(4) after three months, analysis of mechanics and bone density measurement of tibialdefect model were performed. Results:(1) the porosity of the prepared bovine bonescaffold is near to80%，2hours after transplantation, all sheep could stand up and feedthemselves and none of them had life signs instability (2) monthly X-ray test wereperformed to observe the bone formation, after three months the Lane-Sandhu X-ray scoreof autologous red marrow group and the autologous bone graft group was no significantlystatistical differences in bone mineral content between the two groups (P>0.05);(3)therewas also no significantly difference between two groups in biomechanics. Conclusion:(1)xenogeneic bone scaffold porosity prepared by chemical method can conducively createthe canals for the vessels and nerves to grow into;(2) the autologous red marrowcomposite bone is close to autologous bone graft repair the bone defect. |
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