The Experimental Studies Of Repairing Bone Defect With Deep-low-emperature Frozen Allogeneic Bone Combined With Abm (Autologous Bone Marrow) Of Rabbits

Objective: To study repairing segmental bone defects with deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow of rabbits and repair mechanism. Methods: The 15mm segmental bone defects were created on bilateral radius in 36 New Zealand rabbits and were divided into three groups. Group A received deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow, group B: deep-low-temperature-frozen allogeneic bone, group C: autogenous bone. General observation, HE histologic section, serum Alkaline phosphatase, transmission electron microscope and Xray were done in different stages. Results: (1) Three groups were all repaired. (2) Bone formation and synostosis of group A and C surpass group B obviously. (3) Serum Alkaline phosphatase activity of group A and C outstrip group B,P<0.01.(4) Cell accrementition, cell nucleus dissociation, plentiful cytoplasm, nuclear envelope and abundant cell organ of group A and C surpass group B obviously. (5) The bone defects healed in the 8^th week in group A and C, but did not healed in the 10^th week in group B. Conclusion: The deep-low-tempera- turefrozen allogeneic bone combined with autologous bonemarrow of rabbits has good histocom- patibility and osteogenesis; It's possible to repairing bone defects with deep-low-temperature-frozen allogeneic bone combined with autologous bone marrow displacing autogenous bone.