Experimental Studies Of Rats Polyphosphate Biodegradable Nerve Conduits

Objective: Preparation of allograft to nerve scaffolds and cells with poly phosphatecompound, can fall into composite nerve conduit, repair of rat sciatic nerve defect, andstudied the biodegradable composite nerve conduit and pure to cellular neural scaffoldsand autologous nerve transplantation repair effect of the difference, as a substitute forautologous nerve transplantation for its treatment.Methods: For complete rat sciatic nerve of60section, using3%TritonX-100and4%deoxycholic acid sodium solution to repeat extraction into a cell-free nerve scaffolds,1、Take30to cellular neural scaffolds with poly phosphate compound, preparation ofbiodegradable composite nerve scaffolds。2、Take15only biodegradable composite nervescaffold and15simply cellular neural scaffolds for mechanical properties testing。3、45adult male SD rats were randomly divided into three groups, each group of15only,respectively, the experimental group (GroupA), pure decellularized scaffold control(GroupB), autologous transplantation group (GroupC), Unilateral sciatic nerve defectmodel of these three groups make it do the following treatment: group A: biodegradablecomposite decellularized scaffold to repair sciatic nerve defects;Group B: simple cellularneural scaffolds to repair sciatic nerve defects;Group C: autologous sciatic nerve in situimplant. Observation rat hind limbs function recovery after surgery；Made from compositescaffold mechanical testing；Drawn respectively do after march: toluidine blue staincalculation neural axon diameter, number and average size;HE dyeing observation neuralstent axon regeneration;NF-200and schwann cell nucleus immunofluorescence stainingto observe the morphological structure and growth of schwann cells of nerve fibersdistribution;Nerve stent ultrastructure of transmission electron microscope.Results: General observation:1.5months postoperatively, nutrition is not benign changethree group were reduced, regenerated nerve appearance three group were close to normal neural tissue, A, light degree of muscle atrophy group C compared with group B, group Aand C skin sensory and motor function recovery, difference in group B, group CA.Composite scaffold tensile testing and shown its ultimate load and the modulus ofelasticity is superior to pure decellularized scaffold;Regeneration of nerve axon count>group C> group A group B (P <0.05), shows that after two months of group C neural axongrowth significantly more than the other two groups;Axon number, diameter and averagearea> group C> group A group B (P <0.05), number of axons in group C, averagediameter area has restored to A and B groups.HE dye is better than that of group A, groupC is better than that of group B, group A stent connective tissue hyperplasia reshaping,group A is closer to normal neural tissue structure, neural axon diameter coarser, myelinthicker, and group B more connective tissue, usually more obvious;Immunofluorescencetest results: in group A is more positive for the growth of schwann cells and nerve axonmyelin grow better than that of group B, good growth of schwann cells, dense and uniformdistribution, the axon myelin homogeneously zonal distribution of growth, good continuity,is full of internal stent, wave pattern, rules and tidy.While group B schwann cells and nerveaxon myelin grows relatively sparse, uneven distribution, irregular arrangement, thecontinuity is not enough, A, B group of immunofluorescence results than groupC.Transmission electron microscopy (sem) examination results: group A newborn nervefibers is bulky, morphological rules, the arrangement more closely, schwann cellssurrounding the around axons, form A complete myelin, uniform thickness of myelinsheath, the level and group B axon myelin is poorer, axon diameter thickness differenceincreased, irregular arrangement, density is small, myelin wall thickness is not enough, andthickness, the relative difference between group A and group C.Conclusions:1, poly phosphate by3%Triton-100and4%sodium deoxycholic acidextraction of decellularized allogeneic nerve scaffolds can be used as a bridge of nervedefect, no obvious immune rejection.2, compound phosphate ester of biodegradable nervestents cell-free nerve transplantation than simple transplantation has a better performancein physics.3, using compound phosphate of decellularized scaffold to repair peripheralnerve defects may be alternative method of autologous nerve grafts.