The Study Of Dexamethasone Protective Effect On Rat Testicular Torsion Reset And Impact On HO-1and Other Relevant Factors

Objectives:To investigate the effect of dexamethasone in reperfusion injury by rat testiculartorsion; and to explore a sensitive indexes for the clinical early diagnosising inPatient who had Spermatogenesis damage of susceptibility indexes in patientswith spermatogenic impairment of testicular torsion. And provides a certainexperimental and theoretical basis for reducing the testicular damage and protectingthe spermatogenic function in Patient.Method:24male adult SD rats were randomly divided into four groups. The left testiculartorsion reset animal models were established by Turner.Group A: sham group; GroupB: reduction after testicular torsion (torsional reset group); Group C: reduction aftertesticular torsion+NS (torsional reset group+NS); Group D: reduction aftertesticular torsion group+dexamethasone (torsional reset group+dexamethasone).Reverse reset after2hours,and C、D Groups treatment were respectively given salineand dexamethasone(10mg/kg, tail intravenous injection) before the reset30minutes.4hours after operation,the tissue diversification were analyzed by HE stains.Chemocolorimetry were used for evaluation of SOD and MDA in testicular tissue.TUNEL stains and immunohistoche mistry were used for Spermatogenic cellapoptosis and HO-1, respectively.Results:1.Light microscope groups testis HE staining: Compared with group A, therewere more seminiferous tubule degeneration and necrosis, neutrophil infiltration andinterstitial edema the testicular atrophy of the seminiferous tubules in group B andgroup C. Compared with group B, seminiferous tubule structure were stillintact,but slightly smaller in diameter; Only a small amount of seminiferous tubulesnecrosis, neutrophil infiltration and interstitial edema in Group D.2.SOD activity and MDA diversification: Compared with group A（314.12±18.64，1.32±0.33），the SOD activity in B,C,D were246.74±17.15，239.89±16.89， 278.37±10.11，respectively. And MDA diversification in B,C,D were2.65±0.29,2.73±0.491.95±0.41，respectively. The differences were statistically significant(P<0.01). Compared with group B,C, the SOD activity and MDA were increasing ingroup D; the differences were statistically significant(P <0.01). Compared with groupB, however，the differences of SOD activity and MDA diversification were nostatistically significant in group C(P>0.05).3.Cell apoptosis in spermatogenic: Compared with group A（1.46±0.95），the Cellapoptosis AI in B,C,D were17.10±1.51,19.11±1.19,8.70±0.81, respectively. Thedifferences were statistically significant(P <0.01). Compared with group B,C, the Cellapoptosis AI in group D decreased significant (P <0.01). Compared with group B,however，the differences of Cell apoptosis AI were no statistically significant in groupC(P>0.05).4.The Expression of HO-1: Compared with group A（10.56±6.78）, theexpression of HO-1in B,C,D were19394.65±363.36,19649.96±373.59,8584.49±266.93, respectively. And the ascension were statistically significant(P <0.01).Compared with group B,C, the expression of HO-1in group D decreased significant(P <0.05). Compared with group B, however，the differences of HO-1were nostatistically significant in group C(P>0.05).Conclusion:1、Dexamethasone can reduce testicular torsion ischemia-reperfusion injurycaused by twisting. So it can Provide the drug for spermatogenesis damage aftertesticular torsion in Clinical.2、After reset testicular torsion, the expression of H0-1can indirectly reflect thelevel of damage. Therefore,HO-1can be used as an early indicator for testing thelevel of damage after testicular torsion，and can Provide some reference for clinictreatment, simultaneously. But the effective of it need for further investigation.