Basal Ganglia Hemorrhage Impact On Behavioral Development And Dopamine Transporter Of Rat Brain

Objective: To investigate the effects of basal ganglia hemorrhage on behavioraldevelopment and dopamine transporter expression in developmental rats.Methods:1. Rat pups (P10) were randomly divided into cerebral hemorrhage (CH) group, shamoperation (SH) group and normal control (NC) group.2. Chose stereotaxic apparatus inject autologous blood into rats’brain caudate nucleusto establish intracerebral hemorrhage model, SH group injected with nothing, NCgroup received no treatment.3. Used Berderson score to test rats’ neurological functions at the first day, the thirdday, the seventh day, the fourteenth day after operation respectively.4. Used the open-field environment test and the Lat maze to assess behavior of therats at the fourteenth day after operation, testing for three days continuously.5.Used Htoxylin Eosin staining to observe pathological changes and usingimmunohistochemical staining to detect the expression of DAT.Results:1.(1) In open-field test, the number of passed panels, straightening(times) andgrooming(times) in cerebral hemorrhage group were more than those in the shamoperation group and normal control group separately (P <0.05);(2) In the Lat maze,the frequency of running across the corner, straightening (times) and incliming to wall(times) in cerebral hemorrhage group were more than those in the sham operationgroup and normal control group separately (P <0.05).2.H.E staining showed that CH group hematoma formation seen at a small cavity,showing little lymphocytic infiltration and a little glial scar formation; SH groupshowed no hematoma, no cavities formed; NC group no inflammatory cellinfiltration, cell distribution is normal. 4. In the immunohistochemistry study, the integrated optical density(IOD) of DAT inbasal ganglia of rats in the cerebral hemorrhage group were were more than those inthe sham operation group and normal control group separately (P <0.05); while theSH group with no statistical difference between the NC group significantly (P>0.05).5.There was a positive correlation between the basal ganglia DAT and the number ofpassed panels, straightening(times) and grooming(times) in open-field test, and thefrequency of running across the corner, the times of straightening, incliming to wall inthe Lat maze, which with statistical significance (P <0.05).Conclusion:1. Developmental rats focal hemorrhage in basal ganglia is repairable andcompensatory strong, which no more left severe neurological dysfunction.2.The never injury which caused by basal ganglia hemorrhage can lead to autonomicactivity increased, the level of non-selective attention decreased,hyperactivity,attention deficit and other long-term behavioral abnormalities.3Brain dopamine transporter (DAT) expression level increased with developmentalrats in basal ganglia injury which presented hyperactivity, attention deficit and otherdevelopment related to abnormal behavior.