The Influence Of CD133+CXCR4+Colorectal Cancer Cells On The Hepatic Metastasis Formation In Nude Mice

Objective: To observe the expression of CD133and CXCR4in HCT116cell line. Toinvestigate the biological characteristics of CD133+CXCR4+colorectal cancer cellsand investigate the influence of it on the hepatic metastasis of colorectal cancer.Methods:①The expression of CD133and CXCR4in HCT116cell line were analyzedby FACS. To sort the CXCR4+CD133+、CXCR4-CD133-、CXCR4+CD133-、CXCR4-CD133+phenotypic tumor cell from HCT116cell line by FACS,24nudemice were randomly divided into4groups.5×105phenotypic tumor cells wereinjected into spleen to establish nude mice hepatic metastasis models. Inspect thegrowth condition of mice models. All the mice were sacrificed after eight weeks, thehepatic metastases and spleen primary tumor of mice was evaluated respectively.③Wesorted the CXCR4+CD133+、CXCR4-CD133+phenotypic tumor cells from HCT116cell line by FACS，and then18nude mice were randomly divided into3groups:CXCR4+CD133+cells+AMD3100, CXCR4+CD133+cells+PBS, CD133+CXCR4-cells+PBS,5×105phenotypic tumor cells were injected into spleen and then excisespleen to establish nude mice postoperative hepatic metastasis models. To observe thegrowth condition of mice after models were established. All the mice were sacrificedafter45days, the live metastases of each mice were evaluated respectively.Results:(1)Flow cytometry showed that HCT116cell expressed CD133、CXCR4and contained CD133+CXCR4+phenotypic tumor cell.(2) There was no significantdifference of spleen tumor formation rate between CD133+CXCR4+group(6/6) andCD133+CXCR4-group(5/6)(p>0.05), while CD133+CXCR4+group was higher thanthat in CD133-CXCR4+group and CD133-CXCR4-group (p<0.05). What more, therate of spleen tumor formation in CD133+CXCR4-group was higher than that inCD133-CXCR4-group (p<0.05) and it had no difference than that in CD133-CXCR4+group (p>0.05). The incidences of liver metastasis in CD133+CXCR4+group was higher than that in the other three groups (p<0.05). There was no significant differenceamong the other three groups of it (p>0.05).(3) In the assay of antagonist, theincidences of liver metastasis in CXCR4+CD133+cells+AMD3100group was lowerthan that in the CXCR4+CD133+cells+PBS group(p<0.05), while it had no differencewith CD133+CXCR4-cells+PBS group(p>0.05).Conclusion:(1) CD133+colorectal cancer cells maybe had the biologicalcharacteristics of cancer stem cells, and which can express CXCR4.(2)CD133+CXCR4+colorectal cancer cells maybe had the biological characteristics ofmigrating cancer stem cells, which had significant influence on mice hepatic metastasisof colorectal cancer.(3) AMD3100, specific antagonist of SFD-1/CXCR4axis, caninhibit hepatic metastasis of mice which induced by CD133+CXCR4colorectal cancercells.