| ObjectiveStudying of Nrf2and HO-1expression in laryngeal carcinoma, and discussingtheir roles in the pathogenesis of laryngeal carcinoma and their application in thediagnosis and prognosis of laryngeal carcinoma, and providing expermental evidencefor new therapeutid targets.MethodsParaffin-embedded sections and clinic datas were from patients with primarylaryngeal squmamous cell carcinoma diagnosed and treated at the department ofotolaryngology in the First Municipal people’s hospital of guangzhou from January2005to December2007.We respectively used Nrf2and HO-1monoclonal antibody todetect their expressions in laryngeal squmamous cell carcinoma byimmunohistochemistry.The SPSS package (17.0) was used for statistical analysis.Results1.The expression of Nrf2, HO-1in laryngeal squamous cell carcinoma and thevocal cords polyp: Nrf2positive staining mainly locate in the tumor cell nucleus,andrarely in the cytoplasm of Vocal cords polyp.Its protein expression had a significantlypositve detection rate of65.6%(41/61) in laryngeal cacinoma tissues,compared withthat of22.7%(5/22) in vocal cord polyp tissues (P <0.05). HO-1positive stainingmainly locate in the tumor cytoplasm.HO-1protein expression had a significantlypositve detection rate of73.8%(45/61) in laryngeal cacinoma tissues,compared withthat of18.2%(4/22) in vocal cord polyp tissues (P <0.05).2.The relation between the expression of Nrf2, HO-1protein and clinicalpathological features of laryngeal carcinoma: The expression of Nrf2was notralated to gender, age, smoking index, tumor lacalization, tumor size, T stageclassification, clinical stage classifization in laryngeal cacinoma(P>0.05). But it wasrelated to histological classification and metastases in cervical nodes in laryngeal cacinoma (P <0.05); The expression of HO-1was related to histological classification,clinical stage classifization and metastases in cervical nodes in laryngeal cacinoma (P<0.05).3.The correlation between the expression of Nrf2, HO-1proteins markedly have apositively correlation in laryngeal carcinoma tissues by the Spearman correlationanalysis (r=0.823, P <0.05).4.Survival analysis showed that the life span of patients with laryngeal carcinomais significantly inverse-correlated with the expression of Nrf2, HO-1proteins (P <0.05). Cox Regression analysis showed that the expression of Nrf2, HO–1wererelated to life span of laryngeal carcinoma patients. TNM stage, histologicalclassification and tumor recurrence are independent factors for the prognosis inlaryngeal carcinoma.Conclusions1. Nrf2and HO-1proteins are increasing expressed in laryngeal cacinoma. Theexpression of Nrf2was related to histological classification and metastases in cervicalnodes in laryngeal cacinoma.The expression of HO-1was related to histologicalclassification, clinical stage classifization and metastases in cervical nodes inlaryngeal cacinoma.There is a positive correlation between the expressions of Nrf2and HO-1protein. Nrf2and HO-1may be carcinogenic factors and markers ofmalignancy in laryngeal carcinoma.2.Survival analysis showed that the short life span of patients with laryngealcarcinoma is significantly related to the increased expression of Nrf2and HO-1proteins,which suggested that Nrf2and HO-1proteins may provide great potentialsin prognosis of laryngeal carcinoma.3.The detection of Nrf2and HO-1may help the diagnosis of laryngealcancinoma.They could be used as judgement indications of the invasion, metastasisand prognosis in laryngeal cancinoma, and provide experimental evidence for genetherapy of laryngeal cancinoma. |
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