The Expression And Significance Of TWSG1in Craniofacial Fibrous Dysplasia

Background：Fibrous dysplasia （FD）,is a non-hereditary congenital bone tissuedisease.Affected men and women generally begin at childhood. the symptoms isusually a longer course,occur in the jaw facial, maxillofacial.FD clinicalmanifestations appears chronic painless and swelling bulge section,which can causesasymmetry facial appearance further.And when FD grown around，it violates adjacenttissue which can be expressed as nasal congestion, purulent nasal discharge,misaligned bite, eyeball displaces with double vision, hearing loss, headache or othersymptoms. Most patients need surgery to remove the lesion,which often resulting inthe appearance of deformity and dysfunction， brings great suffering to the patient,and seriously affect the quality of life of patients after resection.Study the factorsassociated with the occurrence of fibrous dysplasia and development of fibrousdysplasia to find new ways such as non-surgical treatment to reduce the relapse rateafter treatment is of great significance.Objective：By detecting the expression of twisted gastrulation (TWSG1) protein incraniofacial fibrous dysplasia， we analyze the expression level of the age andprognosis factor， and study the performance of TWSG1in craniofacial fibrousdysplasia,which can enhance the understanding of TWSG1gene further, and canprovide a new theoretical basis at the molecular gene therapy level for curingcraniofacial fibrous dysplasia with TWSG1.Methods：The expression of TWSG1in44cases of paraffin embedded FD and10cases ofjaw fracture from traumata were measured by immunohistochemistry throughEnvision,and the corelation analysis with clinical feature of patients. ALLinformations are statistical analysed by using SPSS19.Chi-squared statistic was usedto analyze statistical significance. Differences were considered statistically explicitmeaning at p<0.05. Results1.44cases express positive in all44.The percentage of TWSG1positiveexpression was in FD tissues(100%) in comparison with normal tissues (20%).Scattered in the osteoblasts and fibroblasts around. Located in thecytoplasm/cytomembrane.2. The experiment figures out TWSG1protein’s expression in FD lesion,andshows the relationship about age differences and prognosis in Table1.TWSG1proteinin FD lesion are all positive, which is higher than in the negative control group ofnormal mandible significantly （P＜0.05）.The TWSG1expression in FD wassignificantly higher in the minor than in adult patients（P＜0.05）.Classify byprognosis,which is lower in the patients with favourable prognosis than theunfavorable ones（P＜0.05）.ConclusionThe expression of TWSG1protein in craniofacial fibrous dysplasia of bonelesion rises, And TWSG1may promote the occurrence and development ofmaxillofacial ossifying fibroma, and which may play an important role in. Theexpression levels of TWSG1in FD lesion is closely related to the developmentprocess of the disease.FD is an important biological indicator of unfavorableprognosis.