| Objective:The aims of this study were to investigate the changes of HMGB-1in serum anddifferent parts of brains of young rats with epilepsy(EP) induced bypentylenetetrazol(PTZ), and to evaluate the relation between HMGB-1and epilepsy.Methods:1. The establishment of chronic epilepsy model.Thirty-two SD male rats were divided into control group (group A, n=8) andexperimental group (n=24) randomly. The control group rats were injectedintra-peritoneally with saline, and the experimental group rats were injectedintra-peritoneally with PTZ by different concentrations, among which theconcentration of group B was20mg·kg-1, group C was40mg·kg-1and group D was60mg·kg-1. After injection, the changes of rats’ behavior were observed at least one hour.The days of kindling required, the latent time, the number of kindling and survival alsoshould be recorded.2. The expression of HMGB-1in different brain regions of rats with epilepsy inducedby PTZ.Forty SD male rats were randomly divided into control group (group A, n=8) andexperimental group (n=32) and they were injected intra-peritoneally with PTZ(40mg·kg-1) and normal saline respectively. According to the time after seizures, the ratswere decapitated at1h(group B),6h(group C),24h(group D)and72h(group E). Thefrontal cortex, hippocampus and mesencephalon were collected, and the levels ofHMGB-1and HMGB-1mRNA in different parts of brain were detected byimmunohistochemistry（IH）and real-time PCR(RT-PCR).Results：1. When the concentration of PTZ was20mg·kg-1, it is hard to kindle. The kindling became violent when the concentration of PTZ was60mg·kg-1, and the progress wasquick and the mortality rate of rats was higher. When the concentration of PTZ was40mg·kg-1, the seizure progress became aggravate gradually,the rate of the kindlingwas higher, and so was the survival.2. The mRNA expression of HMGB-1gradually increased over time dynamically infrontal lobe, midbrain and hippocampus, of which the HMGB-1increased at6h infrontal lobe and hippocampus and that showed a highest level at24h, but decreased at72h. It was still higher than that of the control group yet. There were significantstatistical differences. And for the expression of HMGB-1in midbrain, there were noobvious changes among1h,6h, and24h. Meanwhile, the level of HMGB-1is higherthan that of the control group at72h in midbrain,It showed significant difference.3. The immunohistochemical staining of HMGB-1in hippocampus indicated that theexpression of HMGB-1increased dynamically compared with control group. Ofwhich the HMGB-1increased a little at1h after epileptic seizure, but no significantdifferences were observed. The expression of HMGB-1increased at6h、24h andindicated significant statistics differences. Although it decreased at72h, it was higherthan that in the control group. Significant statistics differences were observed betweenthe two groups.Conclusion：1. The ideal concentration of PTZ which was used by intraperitoneal injection forestablishing the model of chronic epilepsy was40mg kg-1.2. The expression of HMGB-1protein increased after epileptic seizure. The HMGB-1protein is relation to cerebral injury induced by epilepsy. |
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