The Study Of Renal Blood Perfusion In GK Rats Using Targeted Contrast Enhanced Ultrasonography

Objective Prepare targeted microbubbles which carry anti-TM (thrombomodulin) monoclonal antibody by biotin-avidin bridge method. Explore the feasibility of kidney developing in vivo animal and the possibility of reflecting early changes of spontaneous diabetic rats (Goto-Kakizaki, GK) kidney perfusion by using targeted microbubbles. Discuss the characteristics of the targeted contrast agent to develop an image. Assess early diabetic vascular endothelial injury, provide experimental evidence for next step therapeutic schedule and a new inspection method for early detection of diabetic kidney damage.Method1. Prepare targeted SonoVue-TM microbubble by attaching anti-TM monoclonal antibody to the surface of ordinary micro-bubble SonoVue by biotin-avidin bridge method and use ultrasonic instrument to evaluate the developing situation of targeted microbubble in vitro.2.Prepare twenty12-week-old male GK rats, divide them into two groups, twenty Wistar rats of the same age also divided into two groups as control, randomly selected10GK rats and10Wistar rats as targeted angiography group. Get the rest10GK rats and Wistar rats as ordinary angiography group, Rapidly inject targeted microbubbles SonoVue-TM or general microbubble SonoVue to the rats via tail vein, dynamically observate the developing situation of the two contrast agents in rats kidneys. Use developing time-intensity curve (time intensity curve, TIC) to analyse rat kidney perfusion characteristics in different groups. Then analyse the similarities and differences of the two types of contrast agents.Results1.Targeted ultrasound microbubble SonoVue-TM was successfully constructed, and it could be used to develop an external image.2.Targeted microbubbles SonoVue-TM enable clear development of experimental rat kidney. The TIC curve shapes of rat kidney of the two groups showed as single apex with steep ascending and slowly descending branch.3. Compared with the control group, the rising slope of the GK rat renal cortex, medulla in targeted angiography group increased (P<0.05); the peak intensity of medulla increased (P<0.05), and the total area under the curve of medulla increased (P <0.05). Compared with control group, the ascending branch of the GK rat in renal cortex, medulla in ordinary angiography group increased (P<0.05). the peak intensity of the curve increased (P<0.05), and the total area under the curve increased (P<0.05). Compared with the ordinary angiography group, the peak of GK rat medullacurve in targeted angiography group intensity increased (P<0.05), and the total area under the curve increased (P<0.05). Conclusion1. Biotin-avidin bridge method can successfully achieve targeted modifying of ultrasound contrast agent microbubbles, Targeted ultrasound microbubble SonoVue-TM can develop an external image.2. Targeted microbubbles SonoVue-TM can make a clear development of experimental rat kidney, its stable performance meet the requirement of ultrasonic observation time limit, and it can reflect early changes of blood perfusion in GK rat kindey.