The Study Of Epithelial Cell Proliferation And Apoptosis In Patients With Obstructive Jaundice

Objective To investigate the characteristics of intestinal epithelial cell proliferation and apoptosis in patients with obstructive jaundice of and reveal the significance of the change.Methods27cases of gastrointestinal operation treated patients, divided into obstructive jaundice group (n=15, experimental group) and non obstructive jaundice group (n=12, control group), were taken jejunal mucosa in patients during surgery.Intestinal epithelial morphology in patients were observed use HE dyeing; TUNEL method for the detection of intestinal mucosal epithelial cell apoptosis activity; Immunohistochemical detection of Ki-67antigen expression, determination of the proliferative activity of intestinal epithelial cells.Results Compared with the control group, the villus gap of small intestine increased and the villus was discontinuity in the test group.There were widespread Small Sag,and a small amount of lint shedding.in them. The mucous glands were sparse and the mucosal atrophy thinning. The Intestinal mucosal injury grading score of the test group (3.73-1.03) than the control group (1.08-0.79) was significantly elevated, the difference was significant (P<0.01). The intestinal epithelial cell proliferation index Ki67in the test group (54.06-23.60) than the control group (225.42-110.44) was significantly lower, the difference was significant (P<0.01). The apoptosis index in test group (38.20-12.16) than the control group (9.67+4.33) increased obviously, the difference was significant (P<0.01).Conclusion Patients with Obstructive jaundice have declined in the activity in intestinal epithelial cell proliferation and increased apoptosis which may be an important mechanism to cause intestinal mucosal barrier dysfunction in patients with. obstructive jaundice.