The Effect Of B7H3Overexpression To The Sensitivtiy Of Docetaxel And¹⁸F-FDG Uptake On Rat Prostate Cancer Cells In Vitro

Objective The subject used the donated RM1-GFP and RM1-GFP/B7H3rat prostatecell to get acquaintance to the effect of B7H3overexpression to the sensitivtiy ofDocetaxel and18F-FDG uptake on androgen insensitive rat prostate cancer cells throughvitro experiments,and assess the feasibility of B7H3as drug molecular target.Methods The four methyl thiazolyl tetrazolium (MTT) assay was used for thedetection of inhibition of two groups of cell growth in different time and drugconcentrations. Flow cytometry was used for the detection of cell cycle reset of this twogroups. Used Western blot to detect the expression of B7H3of the two groupcells.Simultaneously,18F-FDG uptake of two groups of cells in different cell number wasdetermined.Selected the appropriate cell number, then detected the two groups of cells atdifferent concentration and time of docetaxel for18F-FDG uptake. The cell uptakeinhibition rate of each group was calculated by the formula: cell uptake inhibition rate=(1-uptake rate of treated group/uptake rate of control group)×100%. Used Western blotmethod for the determination of two groups of cell’s glucose transporter-1(Glut-1)expression in the two groups of cells before and after dosing.Results Cells in the two groups after the drug was added in the24,48hours wereboth dose and time dependent.Excepted to the concentrations of5nmol/L and10nmol/L in24hours, RM1-GFP and RM1-GFP/B7H3inhibition rates were（16.60±3.20）%v（s17.06±2.15）%and（25.68±2.97）%vs（23.69±2.75）%respectively, had no statisticallysignificant difference,the other two groups of inhibitory rate of concentration of thedifferences were statistically significant(P<0.05), and the RM1-GFP group wassignificantly higher than that of group RM1-GFP/B7H3. Analysis of the two groups ofcells showed a G2/M arrest by flow cytometry, its degree increases with time, and theRM1-GFP group was significantly higher than that of group RM1-GFP/B7H3. The twogroups of cells of24hours and48hours, uptake of18F-FDG was in a dose-dependent manner in different concentrations, and inhibition rate of18F-FDG uptake of RM1-GFPgroup was significantly higher than group RM1-GFP/B7H3in different drugconcentrations.Conclusion B7H3overexpression can significantly increase the androgenindependent prostate cancer cells of RM1mice for docetaxel resistance and uptake for18F-FDG in vitro.It showed its important role in the tumor chemotherapy drugs such asdocetaxel resistant mechanism.Specific mechanism needs further exploration.