Comparison Of The Rat Respiratory Mucosa Ectomesenchymal Stem Cells And Bone Marrow Mesenchymal Stem Cells Biological Characteristics And Neural Differentiation Potential

The aim of this study is to identify rat nasal septum respiratorymucosa-derived mesenchyme stem cells (RM-MSCs) and to compare its neural lineagedifferentiation capacity with bone marrow-derived mesenchyme stem cells (BM-MSCs)after a short period of neural induction culture. The cell morphology was observed withlight microscopy; cell proliferation was assessed by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet razolium bromide (MTT). The characterristics of the cells were evaluatedwith flow cytometry, immunofuorescence, real-time quantitative PCR (RT-PCR), andWestern blotting. The results showed that rat nasal respiratory mucosa contains RM-MSCsthat exhibited similar proliferation rate as BM-MSCs in vitro. Both RT-PCR and Westernblotting analyses demonstrated that RM-MSCs showed higher expression of neural lineagemarkers than BM-MSCs after a short period of neural induction culture, and secretedhigher level of brain-derived neurotrophic factor. RM-MSCs were more amenable todifferentiate into neural oglial cell after a short period of neural induction culture thanBM-MSCs.