| Osteoarthritis (OA) is the most common disease of human movement system, whichwill affect people’s life to a great extent. Though there’re many treatment strategies, itis still not optimal one, with time passing by, patients’ status exacerbate step by step,which will eventually lead to the dysfunction of the joint.Dexamethasone and Chondroitin sulfate can injected together into articular cavity.Dexamethasone have the ability to anti-inflammatory, detumescence and reduce thecapillary permeability, which will alleviate the patient’s level of articular swelling andhave a clearly effect on relieving the symptoms of OA. As the composition of cartilage,chondroitin sulfate play an important role on regulating the internal environment andmaintaining the stability of cartilage.Object:To compare the theraputic efficacy of knee osteoarthritis via intra-articular cavityinjection of dexamethasone alone with combination of chondroitin sulfate.Method:23patients with knee joint osteoarthitis, which randomly divided into observationgroup and control group, while the observation group will be given intra-articularcavity dexamethasone of injection coupling with chondroitin sulfate, and the controlgroup will receive dexamethasone alone. Both two groups are once a week, and a total four weeks.Result:The effect of observation group which inject dexamethasone and chondroitin sulfateis superior to the group of dexamethasone injection alone. In the meantime, long-termapplying dexamethasone will result in higher recurrence rate.Conclusion:Combination dexamethasone and chondroitin sulfate by intra-articular cavityinjection is an effective therapy for OA. Therefore, physicians can add chondroitinsulfate to protect articular cartilage when applying glucocorticoids in clinical. AbstractArticular cartilage is a type of dense connective tissue, it forms the smooth surfaceof diarthrodial joints and makes joints to glide easily with little friction. It consists ofcartilage extracellular matrix and chondrocytes. Articular cartilage contains no nervesor blood vessels. Chondrocyte is highly specialized cell of mesenchymal origin and beresponsible for producing, sustaining and degrading the cartilage extracellular matrix(ECM). Due to no blood vessel, nerve and lymph system, articular cartilage has limitin proliferation, and self-repair after damage, which lead to osteoarthritis (OA). OA isone of the leading causes of disability in the world, there have been many surgicaladvances in the treatment of cartilage injuries, including chemicals, physiotherapy andbiological scaffolds, there seem to be little improvement in the natural progression ofOA. Researches of mechanism and influence factors of articular cartilage woundhealing are very impotant for prevention and treatment of OA.Chondrocyte can secret various types of collagen and plays a great role inmaintaining the normal function of cartilage tissue. Among various types of collagen,type II collagen is phenotype of hyaline cartilage cell, which can represent80-85%ofthe total collagen. There are reports that chondrocyte will differentiate intofibroblast-like cell with increasing cultivation time. During differentiation, the normalfunction of chondrocyte will be lost, together with the increased expression of type Icollagen and decreased expression of type II collagen. Therefore, research on variation of collagen will reflect the effect of external stimulus on chondrocytes, these externalstimulus include chemicals, fluid shear stress, X ray and so on.Dexamethasone of a certain concentration is capable of inhibiting the proliferationof chondrocyte, it has a trend of up-regulated expression of type I collagen anddown-regulated expression of type II collagen and accelerates the process of cellsfibrosis in the meantime.Chondroitin sulfate (CS) is an element of acid mucopolysaccharide from animaltissue, CS can significantly inhibit the synthesis of matrix metalloproteinase-3(MMP-3), MMP-9and interleukin1β (IL-1β), which decreases expression of type Icollagen and increases expression of type II collagen. Currently, there is no testreported whether CS can relieve dexamethasone inhibition on chondrocytes whendexamethasone and CS exert a combined impact on the cells.Objectives:This study aims to estimate the function of combined chondroitin sulfateanddexamethasone on chondrocyte wound healing under automatic-fabricated scratch.Methods:1. Primary culture of rat chondrocyte and identification.2. Automatic scratch platform establishment.3. Chondrocyte wound healing process in real-time.4. Counting the number of cartilage cells in the same area to determine the optimalconcentration of chondroitin sulfate w h i c h c a n i m p r o v e scratchchondrocytes to heal.5. Applying live/dead kit staining method to identify survival rate and living ofchondrocytes.6. Setting up different groups: scratch D-CS-, scratch CS+, scratch D+, and scratchD+CS+. After culture, collagen I and collagen II were identified byimmunofluorescent technique. Results：1. Obtaining8channels of scratch, and measuring the starting point, terminal point andwidth.2.200μg/ml CS significantly improves the chondrocyte to close the scratch.3. In combined group of dexamethasone and CS, the inhibition of dexamethasone onchondrocytes proliferation can be reduced by CS, which increased expression ofcollagen II and decreased expression of collagen I.Conclusions:1. CS can effectively improve the wound healing of chondrocytes, especially atconcentration of200μg/ml.2. CS can effectively improve the wound healing of cartilage injury caused byglucocorticoid. In clinic, the combined use of glucocorticoid and CS could effectivelykeep articular cartilage from damaging. |
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