Biocompatibility Of Polycaprolactone/Chitosan Electrospun Nanofiber Scaffold And Poly(Lactic-co-glycolic Acid)/Chitosan Electrospun Nanofiber Scaffold Cultured With Schwann Cells

Objective: Using FK506-chitosan conduits treat median nerve defects, verify thesafety of the product and the prognosis of curative effect.Methods:From January2010to January2013No.1Affiliated Hospital of DalianMedical Hand Microsurgery treated9patients whose median nerve were injuried withFK506-chitosan conduits surgery.Surgical indications, including the occurrence of themedian nerve completely amputated, defects in patients with10mm-30mm degree.Patients were followed up for3months to18months. Record start feeling movement bysurgical wound healing and recovery time level; BMRC motor and sensory evaluation;two-point discrimination and hand muscles form.Results: The wound which after the surgery with FK506-chitosan conduits wereallⅠ/Grade healing, local non-allergic and inflammatory reactions,motor and sensoryrecovery times are about four weeks. Postoperative follow-up was77.78%BMRC,excellent in5cases, good in2cases,2cases. Two-point discrimination>5mmaccounted for77.78%of the patients. There was no significant postoperative thenarmuscle atrophy.Conclusion:FK506-chitosan conduits is safe and nontoxic.Using FK506-chitosanconduits surgical treat10mm-30mm median nerve defect,the postoperative recoveryof limb motor and sensory function are satisfactory. Background： With the development of nerve tissue engineering, goodbiocompatibility and degradability of blend copolymerization scaffold materials withseed cells now become a hot research to repair nerve defect. Using electrospinningtechnology spinning blend copolymerization scaffolds, retained the higher porosity andspecific surface area, improve the mechanical strength, hydrophilicity and degradationspeed which are the shortages totraditional single scaffold materials,and improve thebiocompatibility and seed cells.Objective:Using electrospinning technology spinningpolycaprolacton(PCL)/chitosan(CS) electrospun nanofiber scaffold andpoly(lactic-co-lycolic acid)(PLGA)/chitosan(CS) electrospun nanofiberscaffold.Evaluating their mechanical property,porosity and hydrophilicproperty.Comparing biocompatibility of two kinds of nanofiber scaffold,which culturedwith schwann cells(SCs).Methods:Using electrospinning technology spinning PCL/CS electrospunnanofiber scaffold and PLGA/CS electrospun nanofiber scaffold;Dissecting both sidesof SD rats sciatic nerve, separating and culturing SCs in vitro;identifying SCs byimmunocytochemical S-100staining;cck-8assay detect cell viabilities of SCs culturedin the extractions of both scaffolds,influenced the toxicity of scaffold materials; SCsco-cultured with two of the nanofibers scaffold,using Inverted microscope, fluorescencemicroscope and scanning electron microscope observe the growth situation; detectingthe apoptosis rate by flow cytometry,cell adhesion rate by cell counted and cell proliferation rate by cck-8assay.Resμlts:Both PCL/CS electrospun nanofiber scaffold and PLGA/CS electrospunnanofiber scaffold have good quality of mechanical property,porosity and hydrophilicproperty;Success in vitro separation and production pure SCs;By the observation ofinverted microscope, immunocytochemical S-100staining and scanning electronmicroscope,SCs growing well in clusters on both of scaffolds, at full stretch, andintegrating with nanofibers;Two kinds of scaffold materials are non-toxic；both the SCsapoptosis rate cultured on two kinds of scaffolds under10%，same with blank controlgroup(p>0.05);The adhesion rate of SCs on PLGA/CS are higher than PCL/CS groupand blank control group(p<0.05),PCL/CS group’s and blank control group’s aresimilar(p>0.05);The proliferation rate of SCs on PLGA/CS（129.09%） are higher thanPCL/CS group’s （101.41%）(p<0.05),PCL/CS group’s and blank control group’s aresimilar(p>0.05).Conclusion:Both PCL/CS electrospun nanofiber scaffold and PLGA/CSelectrospun nanofiber scaffold have good quality of mechanical property,porosity andhydrophilic property,and non-toxic;For the growth of SCs,two kinds of scaffoldsprovides a good3d growth environment, have good biocompatibility;The adhesion rateand proliferation rate of SCs on PLGA/CS are higher than PCL/CS group’s.