| Part One Rabbits’Abdominal Aorta Atheromatous Plaque Model Establishment and Intervention effectObjective:Using pure high-fat feeding rabbits to set up abdominal aorta atheromatous plaque model, to investigate the relationship between the blood lipid levels and the rabbit abdominal aorta atheromatous plaque, and at the same time we observed the intervention effect of atorvastatin to rabbits’ abdominal aorta atheromatous plaque.Method:1. Establishing the rabbits’abdominal aorta atheromatous plaque model:Divided the30of male Japanese big ear rabbits randomly into three groups,10for each groups, they were respectively the control group(group N), the model of non-intervention group (group M) and the atorvastatin intervention group(group A). the model of non-intervention group and the atorvastatin intervention group received high fat feeding(The recipe is2%total cholesterol,8%yolk powder,10%beef tallow,5%white sugar,75%ordinary fodder), feeding the control group with the ordinary fodder,100g/day,feeding time is16weeks.2.Atorvastatin interventionhe:atorvastatin intervention group high-fat feeding after1week, started using atorvastatin calcium tablet, dose of5mg/Kg/day to fill the stomach, feeding time is16weeks. 3.Monitoring of three experimental blood lipid level, compare the three experimental week0,8,16weekend weekend of TC, TG level and the change trend, atorvastatin on lipid intervention effect.4. Compare the three experiment’s blood lipid level, abdominal aorta IMT and the situation of the atheromatous plaque, and then we observed the intervention effect of atorvastatin to the rabbits’abdominal aorta atheromatous plaque.5.Rabbit abdominal aorta atheromatous plaque contrast ultrasound and pathology results, two-dimension ultrasound for the evaluation of atheromatous plaque diagnostic sensitivity.Result:LPure high-fat feeding Japanese big ear rabbit after16weeks, by ultrasound and pathological results confirm rabbit abdominal aorta atheromatous plaque formation, explains the success of the experiment to establish the rabbit model of abdominal aortic atherosclerotic.2. Compare each week blood specimens indicators:control group in0,8,16weeks between serum total cholesterol (TC), triglyceride (TG) no significant difference (P>0.05).16weekend, model of intervention group and atorvastatin intervention group serum total cholesterol (TC) and triglyceride (TG) level is significantly higher than0and8weekend weekend the intervention group (P<0.05), a model of serum total cholesterol and triglyceride and higher than atorvastatin intervention group (P<0.05).3.The TC and TG of rabbits with increasing feeding time, but atorvastatin intervention group compared with model of intervention group blood fat rise gently, shows that atorvastatin has certain fall hematic fat action.4. Comparing the different experimental groups’ intima-media thickneas(IMT) at different times, the model nont-ervention group and the intervention atorvastatin group’s intima-media thickness is higher than the control group. There are differences in the intima-media thickneas between the model nont-ervention group and the atorvastatin intervention group(P<0.05), the intima-media thickneas is thinner in the atorvastatin group than in the control group. 5.In the16weekend, the models of non-intervention group were found17plaques. Atorvastatin intervention group were found11plaques. That atorvastatin has antagonism atheromatous plaque formation.6.Compared with two-dimensional ultrasound by pathological examination, model of inon-ntervention group for missing three patches., atorvastatin intervention group for missing two patches.Conclusion:1. Through pure high-fat feeding method to establish the rabbit model of abdominal aorta atheromatous plaque build successful, there is a close relationship with lipid levels of atherosclerosis.2. The establishment of the model of abdominal aorta and the level of blood fat has intimate connection.3. Hyperlipidemia effect the thickness of the abdominal aortic intima-media(IMT) and the formation of the atheromatous plaque. Atorvastatin has the function of anti-hyperlipidemia and reduce the intima-media thickening in the high fat state, and has the function of reduce plaque formation and stable the plaque.4.The pathological results confirmed that the two-dimensional ultrasound lining part of the abdominal aorta is localized thickening display in atheromatous plaque under the mirror, that two-dimensional ultrasound for weak echo plaques showed is not clear, easily missed diagnosis. Part two The Experiment Research of Nanoscale Ultrasound Contrast Agent to Rabbits’Abdominal Aorta Atheromatous PlaqueObjective:Using self-made nanometer contrast agents and SonoVue contrast agent to proceed ultrasound contrast, and then explore the imaging effect of self-made nanometer contrast agents to the atheromatous plaque.Method:1.High-fat feeding rabbit abdominal aorta atheromatous plaque model is set up: Methods with former.2.Making the self-made nanometer nanoscale contrast agents,and SonoVue contrast agent。3. The self-made nanoscale ultrasound contrast agent and acoustic novy contrast agents on abdominal aorta in rabbits imaging contrast research(1)Two-dimensional ultrasonic observation of abdominal aorta.(2)The self-made nanoscale ultrasound contrast agent for12weeks and16weeks model group rabbit abdominal aorta atheromatous plaque imaging analysis and comparative study.(3)The self-made nanoscale ultrasound contrast agent and acoustic novy contrast agent (SonoVue) for16weeks model group rabbit abdominal aorta atheromatous plaque imaging contrast research.(4)Using PHLIPIE33diasonograph’s Qlab8.1time intensity analysis software to quantify analyze the these two contrast agents’abdominal aorta atheromatous plaque contrast-enhanced ultrasound images, and at the same time compared with the pathological results.4. The rabbit abdominal aorta atheromatous plaque for pathological study(1)Model group of16weekend rabbit abdominal aorta atheromatous plaque gross observation and results of ultrasonic imaging. (2)the microscopic observation model group16weekend rabbit abdominal aorta atheromatous plaque.Result:1. The self-made belongs to nanoscale microbubble ultrasound contrast agents nanoscale.12weekend of high-fat feeding rabbit abdominal aorta atheromatous plaque imaging experimental results show that the the self-made nanoscale ultrasound contrast agent can enhance the development of plaque, the self-made nanoscale ultrasound contrast agent to make success.2. The self-made nanometer contrast agent’s in vitro experiment imaging of rabbits’abdominal aorta of12week show that the contrast agent first show in tube cavity, and the intima and atheromatous plaque imaging time delay, the echo gradually enhance, the plaque’s echo is significantly enhanced than the adjacent intima, and the boundary is clear compared with angiography, and the circum vasa vasorum shows obviously.3. The self-made nanoscale ultrasound contrast agent in12weeks and16weeks of model group after the rabbit imaging contrast, found in12weeks and16weeks home-made active to rabbit abdomen nanoscale ultrasound contrast agent, in the development of the vascular lumen, intimal both ultrasonic average, peak strength, time, tmax, peak of half time has no obvious difference (P>0.05). For plaques, in12weeks and16weeks of homemade nanoscale ultrasound contrast agent to the emergence of plaque imaging time, tmax, peak of half time compared to no significant difference (P>0.05), and ultrasonic average, peak strength, peak of half time comparison, the difference was statistically significant (P<0.05).4. The self-made nanoscale ultrasound contrast agent and acoustic novy contrast agent for16weeks model group rabbit abdominal aorta atheromatous plaque imaging, lumen, intimal, patches of ultrasonic time, average, peak strength, peak time, peak of half time have obvious difference (P<0.05), the self-made nanoscale ultrasound contrast agent and acoustic novy contrast agents in vascular cavity development there is a difference.5. For the development of the plaques, homemade nanoscale ultrasound contrast agent compared with sound novy contrast agents, ultrasonic acoustic novy contrast agents are less than average, peak strength, but the time development faster than sound novy contrast agents, peak time later than sound novy contrast agents, half peak time is longer than sound novy contrast agents.6.High-fat feeding last to the end of the16week and7rabbits left(2suffer accidental death), the two-dimensional ultrasonography can find17atheromatous plaques, among which6hypoecho are mainly soft plaques,7equal echo,4strong echo, which means the high-fat animal model is successful established. The self-made nanometer contrast agent detect the plaque which the two-dimensional ultrasonography do not find, the SonoVu contrast agent find2plaque which the two-dimensional ultrasonography missed diagnosis.Conclusion:1. The diameter of the self-made nanometer contrast agent’s micro bubble belong to the nanometermeter level, and at the rabbits’abdominal aorta atheromatous plaque show can enhance the imaging of the plaque, which means the nanometer contrast agent is successfully made.2. The self-made nanometer contrast agent of this experiment and the SonoVu contrast agent all have the function of strengthen the plaque’s imaging, and at the same time combine longer with the plaque, which is more easy to identify the plaque.3. The self-made nanometer contrast agent can enhance the imaging of the rabbits’ abdominal aorta atheromatous plaque.4. Two-dimensional ultrasound for weak echo plaques showed is not clear, easily missed diagnosis.The ultrasound contrast can find the plaque which the two-dimensional ultrasound is not easy to detect, and have more sensibility to the diagnose of the plaque. |
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